Targeting Pathogenic Bacteria

Canadian researchers develop a microresonator array to detect specific bacteria

Article ID: 664147

Released: 3-Nov-2016 9:00 AM EDT

Source Newsroom: AVS: Science and Technology of Materials, Interfaces, and Processing

From the 63rd International Symposium and Exhibition of AVS

Newswise — Washington, D. C., November 09, 2016—Bacterial pathogens pose serious health risks, especially for infants, young children, elderly and those with compromised immune systems. The evolution of drug-resistant bacteria is particularly concerning in the fight against disease. A research team in Canada is exploring a new platform for detecting pathogenic bacteria using bacteriophages, viruses that use bacteria as their host.

During the AVS 63rd International Symposium and Exhibition being held November 6-11, 2016, in Nashville, Tennessee, Stephane Evoy, an applied physicist from the University of Alberta, will explain how the team recognized the limited reliability of antibodies in providing bacteria detection with specificity. Instead they used phage-derived proteins, proteins developed from the bacteria-invading viruses, for detection of pathogenic bacteria to address this deficiency. This work has implications not only in disease diagnosis, but also in food and water safety.

“The high specificity of phages offers a potent alternative for the targeting of pathogens," Evoy said. "More specifically, recombinant phage-receptor-binding proteins (RBPs) responsible for phage-host specificity can be used as biological probes and present numerous advantages over the use of a whole phage."

The study used skim cow milk spiked with different phages or combinations of phages, such as mycobacteria (MAP) and Escherichia coli cells, and a unique process to capture the DNA after incubation. The entire process took less than 24 hours and resulted in significantly better sensitivity of detecting targeted DNA.

“The use of phage-derived proteins in such a manner was quite unique when we started that work back in 2005, but since then the approach thrived, and multinational companies integrated this into their product line,” Envoy said. “However, there is still a lot of work to be done in terms of applying the technology to diseases such as tuberculosis and staphylococcus infections.”

In addition to demonstrating this capture technique, the research team designed and developed a sophisticated bacteria detector comprised of an array of microresonators, able to enumerate bacteria over a large area and detect the attachment of a single cell anywhere on the array. The devices were prepared with their phage proteins, adding this high specificity of detection to the spatial precision offered by the array design.

“We are looking forward to adapting this technology for the rapid diagnosis of drug-resistant bacteria,” Evoy said. “It could go a long way toward make microbial testing methods both more rapid and affordable.”

###Presentation BI+MI-WeM1, "Bacteriophage-Derived Surfaces for the Targeting of Pathogenic Bacteria," is at 8:00 a.m. CDT, Nov. 9, 2016, in Room 101BA MORE ABOUT THE AVS 63rd INTERNATIONAL SYMPOSIUM & EXHIBITIONThe symposium is being held November 6-11, 2016, in Nashville, Tennessee

USEFUL LINKSMain symposium website: https://www.avs.org/SymposiumTechnical Program: http://www.avssymposium.orgMedia Center: https://www.avs.org/About/Press-Media-Center

PRESSROOMTo request free press registration, please contact Della Miller at della@avs.org.

ABOUT AVS As an interdisciplinary, professional Society, AVS supports networking among academic, industrial, government, and consulting professionals involved in a variety of disciplines - chemistry, physics, biology, mathematics, all engineering disciplines, business, sales, etc. through common interests related to the basic science, technology development, and commercialization of materials, interfaces, and processing area.

Founded in 1953, AVS is organized into technical divisions and technical groups that encompass a range of established as well as emerging science and technology areas. There are also regional chapters, international chapters and affiliates, and student chapters that promote communication and networking for professionals and students within a geographical region. AVS is comprised of approximately 4,500 members worldwide.

AVS is a member society of the American Institute of Physics with additional benefits for our members. For more information about AVS, visit our website at http://www.avs.org


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