Doe Science news source
The DOE Science News Source is a Newswise initiative to promote research news from the Office of Science of the DOE to the public and news media.
  • 2018-03-07 14:05:54
  • Article ID: 690742

With Laser Light, Scientists Create First X-Ray Holographic Images of Viruses

Using SLAC's X-ray laser, researchers have made the detailed 3-D images of nanoscale biology, with future applications in the study of air pollution, combustion and catalytic processes.

  • Credit: Anatoli Ulmer and Taisia Gorkhover / The Technical University of Berlin and SLAC National Accelerator Laboratory

    This image shows simulated X-ray diffraction patterns that form an in-flight hologram. The depth information is captured from the curved lines.

  • Credit: Anatoli Ulmer and Taisia Gorkhover / The Technical University of Berlin and SLAC National Accelerator Laboratory

    In the new study, the authors superimposed scattered X-ray light from the mimivirus with scattered X-ray light from a reference sphere (main image). The curvature in the superimposed images from the two objects provided depth information and details about the shape of the virus. The image in the lower right corner is a holographic reconstruction of the virus based on the X-ray diffraction patterns collected during the experiment.

  • Credit: Anatoli Ulmer and Taisia Gorkhover / The Technical University of Berlin and SLAC National Accelerator Laboratory

    Illustration showing the principle of in-flight holography. (Left) The X-rays scatter off two spheres and form a characteristic diffraction pattern. The patterns are recorded using the very intense X-ray beam of SLAC’s X-ray laser, the Linac Coherent Light Source (LCLS). (Center) Changes in size and distance of the spheres are reflected in the patterns which can be directly translated from the diffraction alone. The smaller sphere can act as a holographic reference. (Right) If the spheres are shifted out of plane, the fine lines of the diffraction pattern become curved. The signatures of the position and size of the reference allow researchers to reconstruct the 3-D distances between the small sphere (reference) and the large sphere.

Holography, like photography, is a way to record the world around us. Both use light to make recordings, but instead of two-dimensional photos, holograms reproduce three-dimensional shapes. The shape is inferred from the patterns that form after light ricochets off an object and interferes with another light wave that serves as a reference.

When created with X-ray light, holography can be an extremely useful method for capturing high-resolution images of a nanoscale object—something that is so small, its size is measured in nanometers, or billionths of a meter.

So far, X-ray holography has been restricted to objects that form crystals or relied on careful positioning of the sample on a surface. However, many nano-sized particles are non-crystalline, short-lived and very fragile. They may also suffer changes or damage during an experiment when positioned on a surface. Aerosols, exotic states of matter, and the smallest forms of life often fall into these categories and therefore are difficult to study with conventional imaging methods.

In a recent study featured on the March 2018 cover of Nature Photonics, researchers developed a new holographic method called in-flight holography. With this method, they were able to demonstrate the first X-ray holograms of nano-sized viruses that were not attached to any surface.

The patterns needed to create the images were taken at the Linac Coherent Light Source (LCLS), the X-ray free-electron laser at the Department of Energy’s SLAC National Accelerator Laboratory. Nanoviruses have been studied at LCLS without a holographic reference, but the interpretation of the X-ray images required many steps, relied on human input and was a computationally challenging task.

In the new study, the authors superimposed scattered X-ray light from the virus with scattered X-ray light from a reference nano-sized sphere. The curvature in the superimposed images from the two objects provided depth information and details about the shape of the 450-nanometer-wide virus, the mimivirus. This technique greatly simplified the interpretation of the data.

“Instead of thousands of steps and algorithms that potentially don’t match up, you have a two-step procedure where you clearly get the structure out of your image,” says lead study author Tais Gorkhover, a Panofsky Fellowat SLAC and researcher at the Stanford PULSE Institute.

Now, the scientists can do their reconstruction of a sample in fractions of a second or even faster with the holographic method.

“Before our study, the interpretation of the X-ray images was very complicated and the structure of nanosamples was reconstructed long after the actual experiment using non-trivial algorithms,” says Christoph Bostedt, a scientist at the DOE’s Argonne National Laboratory and a co-author of the study. “With ‘in-flight’ holography, the procedure is very simple and in principle can be performed while taking data. This is a real breakthrough.”

“Another advantage of the in-flight holography method is that it is less prone to noise and to the artifacts that can appear in the detector compared to non-holographic X-ray imaging,” says Anatoli Ulmer, a co-author and PhD student from the Technical University of Berlin in Germany.

In the long run, the researchers predict that in-flight holography will offer new ways to study air pollution, combustion and catalytic processes, all of which involve nanoparticles.

An international team of scientists from the DOE’s Lawrence Berkeley and Brookhaven national laboratories, the Chalmers University of Technology in Sweden, the Czech Academy of Science, Deutsches Elektronen-Synchrotron (DESY) in Germany, the Institute of Optics and Atomic Physics in Germany, the Institute for Solid State Physics and Optics in Hungary, KTH Royal Institute of Technology in Sweden, Northwestern University and Uppsala University in Sweden also contributed to this research.

LCLS is a DOE Office of Science user facility.

SLAC is a multi-program laboratory exploring frontier questions in photon science, astrophysics, particle physics and accelerator research. Located in Menlo Park, Calif., SLAC is operated by Stanford University for the U.S. Department of Energy's Office of Science. For more informatiom, please visit slac.stanford.edu.

SLAC National Accelerator Laboratory is supported by the Office of Science of the U.S. Department of Energy. The Office of Science is the single largest supporter of basic research in the physical sciences in the United States, and is working to address some of the most pressing challenges of our time. For more information, please visit science.energy.gov.

X
X
X
  • Filters

  • × Clear Filters

Is Nature Exclusively Left Handed? Using Chilled Atoms to Find Out

Elegant techniques of trapping and polarizing atoms open vistas for beta-decay tests of fundamental symmetries, key to understanding the most basic forces and particles constituting our universe.

Forever Young Catalyst Reduces Diesel Emissions

Atom probe tomography reveals key explanations for stable performance over a cutting-edge diesel-exhaust catalyst's lifetime.

Sense Like a Shark: Saltwater-Submersible Films

A nickelate thin film senses electric field changes analogous to the electroreception sensing organ in sharks, which detects the bioelectric fields of prey.

Enhanced Detection of Nuclear Events, Thanks to Deep Learning

Scientists at Pacific Northwest National Laboratory are exploring deep learning to interpret data related to national security, the environment, the cosmos, and breast cancer. In one project a deep neural network is interpreting data about nuclear events as well as - sometimes better than - today's best automated methods or human experts.

A Bit of Quantum Logic--What Did the Atom Say to the Quantum Dot?

Let's talk! Scientists demonstrate coherent coupling between a quantum dot and a donor atom in silicon, vital for moving information inside quantum computers.

Sodium- and Potassium-based Batteries Hold Promise for Cheap Energy Storage

Researchers at the Georgia Institute of Technology have found new evidence suggesting that batteries based on sodium and potassium hold promise as a potential alternative to lithium-based batteries.

ORNL researchers use AI to improve mammogram interpretation

In an effort to reduce errors in the analyses of diagnostic images by health professionals, a team of researchers from Oak Ridge National Laboratory has improved understanding of the cognitive processes involved in image interpretation, work that has enormous potential to improve health outcomes for the hundreds of thousands of American women affected by breast cancer each year. The ORNL-led team found that analyses of mammograms by radiologists were significantly influenced by context bias, or the radiologist's previous diagnostic experiences.

Scientists Make the First Molecular Movie of One of Nature's Most Widely Used Light Sensors

Scientists have made the first molecular movie of the instant when light hits a sensor that's widely used in nature for probing the environment and harvesting energy from light. The sensor, a form of vitamin A known as retinal, is central to a number of important light-driven processes in people, animals, microbes and algae, including human vision and some forms of photosynthesis, and the movie shows it changing shape in a trillionth of an eye blink.

Scientists isolate protein data from the tiniest of caches - single human cells

Scientists have captured the most information yet about proteins within a single human cell, giving scientists one of their clearest looks yet at the molecular happenings inside a human cell. The team detected on average more than 650 proteins in each cell - many times more than conventional techniques capture from single cells.

Researchers Generate Electricity and Hydrogen from Live Bacteria

Using a family of photosynthetic bacteria that commonly live in lakes and seas, researchers at the Technion have developed a technology to generate electricity and hydrogen energy. The researchers believe their technology can serve as a promising source of clean, environment-friendly energy that will not emit pollutants during production or use (hydrogen fuel).


  • Filters

  • × Clear Filters

Department of Energy Selects 84 Scientists to Receive Early Career Research Program Funding

The Department of Energy (DOE) has selected 84 scientists from across the nation - including 30 from DOE's national laboratories and 54 from U.S. universities - to receive significant funding for research as part of the DOE Office of Science's Early Career Research Program.

Three Argonne Scientists Receive DOE Early Career Awards

Three Argonne researchers have earned the DOE's 2018 Early Career Research Program awards.

Four SLAC Scientists Awarded Prestigious DOE Early Career Research Grants

Four scientists at the Department of Energy's SLAC National Accelerator Laboratory will receive Early Career Research Program awards for research that's developing new ways to study fundamental particles with machine learning and study nanoscale objects and quantum materials with powerful X-ray laser beams.

PPPL Physicists Aim to Unlock Mysteries of Fusion with Early Career Research Awards

Article describes PPPL winner of DOE Early Career Awards.

Ames Laboratory's Ke Earns DOE Early Career Research Award

Ames Laboratory scientist Liqin Ke is one of 30 scientists from the U.S. Department of Energy's (DOE) national laboratories to be selected for funding as part of the DOE's Early Career Research Program.

6 Berkeley Lab Researchers Receive DOE Early Career Research Awards

Six scientists at Lawrence Berkeley National Laboratory (Berkeley Lab) have been selected by the U.S. Department of Energy's (DOE's) Office of Science to receive significant funding for research through its Early Career Research Program.

Stony Brook's AERTC Selected for Leadership Role in Nationwide Consortium on Offshore Wind Industry

Stony Brook's Advanced Energy Research and Technology Center (AERTC) will play a key role in a nationwide research and development consortium for the offshore wind industry. The New York State Energy Research and Development Authority was awarded a $18.5 million U.S. Department of Energy grant to establish the National Offshore Wind Research and Development Consortium, Governor Andrew M. Cuomo announced last week. New York State, through NYSERDA, has committed an additional $20.5 million to fund the consortium supporting Gov. Cuomo's initiative Reforming the Energy Vision (REV).

Professor Emily Liu Receives $1.8 Million DoE Award for Solar Power Systems Research

Li (Emily) Liu, associate professor of nuclear engineering and engineering physics in the Department of Mechanical, Aerospace, and Nuclear Engineering at Rensselaer Polytechnic Institute, has been selected by the U.S. Department of Energy Solar Energy Technologies Office (SETO) to receive a $1.8 million award to study high-temperature molten-salt properties and corrosion mechanisms.

Vasilis Fthenakis Receives IEEE's William R. Cherry Award

UPTON, NY; Vasilis Fthenakis, a Senior Scientist Emeritus at the U.S. Department of Energy's (DOE) Brookhaven National Laboratory and Founder and Director of the Center for Life Cycle Analysis at Columbia University, will receive the 2018 William R. Cherry Award from the Institute of Electrical & Electronics Engineers (IEEE).

New PPPL director Steve Cowley is honored with knighthood by Queen Elizabeth II

Steven Cowley, newly named director of the U.S. Department of Energy's (DOE) Princeton Plasma Physics Laboratory (PPPL) effective July 1, has received a knighthood from Queen Elizabeth "for services to science and the development of nuclear fusion."


  • Filters

  • × Clear Filters

Is Nature Exclusively Left Handed? Using Chilled Atoms to Find Out

Elegant techniques of trapping and polarizing atoms open vistas for beta-decay tests of fundamental symmetries, key to understanding the most basic forces and particles constituting our universe.

As Future Batteries, Hybrid Supercapacitors Are Super-Charged

A new supercapacitor could be a competitive alternative to lithium-ion batteries.

Forever Young Catalyst Reduces Diesel Emissions

Atom probe tomography reveals key explanations for stable performance over a cutting-edge diesel-exhaust catalyst's lifetime.

Sense Like a Shark: Saltwater-Submersible Films

A nickelate thin film senses electric field changes analogous to the electroreception sensing organ in sharks, which detects the bioelectric fields of prey.

A Bit of Quantum Logic--What Did the Atom Say to the Quantum Dot?

Let's talk! Scientists demonstrate coherent coupling between a quantum dot and a donor atom in silicon, vital for moving information inside quantum computers.

New Tech Uses Isomeric Beams to Study How and Where the Galaxy Makes One of Its Most Common Elements

A new measurement using a beam of aluminum-26 prepared in a metastable state allows researchers to better understand the creation of the elements in our galaxy.

Simulations of Magnetically Confined Plasmas Reveal a Self-Regulating Stabilizing Mechanism

A mysterious mechanism that prevents instabilities may be similar to the process that maintains the Earth's magnetic field.

Seeing All the Colors of the Plasma Wind

2-D velocity imaging helps fusion researchers understand the role of ion winds (aka flows) in the boundary of tokamak plasmas.

Renewable Solvents Derived From Lignin Lowers Waste in Biofuel Production

New class of solvents breaks down plant biomass into sugars for biofuels and bioproducts in a closed-loop biorefinery concept.

Scientists Studying Nuclear Spin Make a Surprising Discovery

The size of a nucleus appears to influence the direction of certain particles emitted from collisions with spinning protons.


Spotlight

Monday June 18, 2018, 09:55 AM

Creating STEM Knowledge and Innovations to Solve Global Issues Like Water, Food, and Energy

Illinois Mathematics and Science Academy (IMSA)

Friday June 15, 2018, 10:00 AM

Professor Emily Liu Receives $1.8 Million DoE Award for Solar Power Systems Research

Rensselaer Polytechnic Institute (RPI)

Thursday June 07, 2018, 03:05 PM

Celebrating 40 years of empowerment in science

Argonne National Laboratory

Monday May 07, 2018, 10:30 AM

Introducing Graduate Students Across the Globe to Photon Science

Brookhaven National Laboratory

Wednesday May 02, 2018, 04:05 PM

Students from Massachusetts and Washington Win DOE's 28th National Science Bowl(r)

Department of Energy, Office of Science

Thursday April 12, 2018, 07:05 PM

The Race for Young Scientific Minds

Argonne National Laboratory

Wednesday March 14, 2018, 02:05 PM

Q&A: Al Ashley Reflects on His Efforts to Diversify SLAC and Beyond

SLAC National Accelerator Laboratory

Thursday February 15, 2018, 12:05 PM

Insights on Innovation in Energy, Humanitarian Aid Highlight UVA Darden's Net Impact Week

University of Virginia Darden School of Business

Friday February 09, 2018, 11:05 AM

Ivy League Graduate, Writer and Activist with Dyslexia Visits CSUCI to Reframe the Concept of Learning Disabilities

California State University, Channel Islands

Wednesday January 17, 2018, 12:05 PM

Photographer Adam Nadel Selected as Fermilab's New Artist-in-Residence for 2018

Fermi National Accelerator Laboratory (Fermilab)

Wednesday January 17, 2018, 12:05 PM

Fermilab Computing Partners with Argonne, Local Schools for Hour of Code

Fermi National Accelerator Laboratory (Fermilab)

Wednesday December 20, 2017, 01:05 PM

Q&A: Sam Webb Teaches X-Ray Science from a Remote Classroom

SLAC National Accelerator Laboratory

Monday December 18, 2017, 01:05 PM

The Future of Today's Electric Power Systems

Rensselaer Polytechnic Institute (RPI)

Monday December 18, 2017, 12:05 PM

Supporting the Development of Offshore Wind Power Plants

Rensselaer Polytechnic Institute (RPI)

Tuesday October 03, 2017, 01:05 PM

Stairway to Science

Argonne National Laboratory

Thursday September 28, 2017, 12:05 PM

After-School Energy Rush

Argonne National Laboratory

Thursday September 28, 2017, 10:05 AM

Bringing Diversity Into Computational Science Through Student Outreach

Brookhaven National Laboratory

Thursday September 21, 2017, 03:05 PM

From Science to Finance: SLAC Summer Interns Forge New Paths in STEM

SLAC National Accelerator Laboratory

Thursday September 07, 2017, 02:05 PM

Students Discuss 'Cosmic Opportunities' at 45th Annual SLAC Summer Institute

SLAC National Accelerator Laboratory

Thursday August 31, 2017, 05:05 PM

Binghamton University Opens $70 Million Smart Energy Building

Binghamton University, State University of New York

Wednesday August 23, 2017, 05:05 PM

Widening Horizons for High Schoolers with Code

Argonne National Laboratory

Saturday May 20, 2017, 12:05 PM

Rensselaer Polytechnic Institute Graduates Urged to Embrace Change at 211th Commencement

Rensselaer Polytechnic Institute (RPI)

Monday May 15, 2017, 01:05 PM

ORNL, University of Tennessee Launch New Doctoral Program in Data Science

Oak Ridge National Laboratory

Friday April 07, 2017, 11:05 AM

Champions in Science: Profile of Jonathan Kirzner

Department of Energy, Office of Science

Wednesday April 05, 2017, 12:05 PM

High-Schooler Solves College-Level Security Puzzle From Argonne, Sparks Interest in Career

Argonne National Laboratory

Tuesday March 28, 2017, 12:05 PM

Champions in Science: Profile of Jenica Jacobi

Department of Energy, Office of Science

Friday March 24, 2017, 10:40 AM

Great Neck South High School Wins Regional Science Bowl at Brookhaven Lab

Brookhaven National Laboratory

Wednesday February 15, 2017, 04:05 PM

Middle Schoolers Test Their Knowledge at Science Bowl Competition

Argonne National Laboratory

Friday January 27, 2017, 04:00 PM

Haslam Visits ORNL to Highlight State's Role in Discovering Tennessine

Oak Ridge National Laboratory

Tuesday November 08, 2016, 12:05 PM

Internship Program Helps Foster Development of Future Nuclear Scientists

Oak Ridge National Laboratory

Friday May 13, 2016, 04:05 PM

More Than 12,000 Explore Jefferson Lab During April 30 Open House

Thomas Jefferson National Accelerator Facility

Monday April 25, 2016, 05:05 PM

Giving Back to National Science Bowl

Ames Laboratory

Friday March 25, 2016, 12:05 PM

NMSU Undergrad Tackles 3D Particle Scattering Animations After Receiving JSA Research Assistantship

Thomas Jefferson National Accelerator Facility

Tuesday February 02, 2016, 10:05 AM

Shannon Greco: A Self-Described "STEM Education Zealot"

Princeton Plasma Physics Laboratory

Monday November 16, 2015, 04:05 PM

Rare Earths for Life: An 85th Birthday Visit with Mr. Rare Earth

Ames Laboratory

Tuesday October 20, 2015, 01:05 PM

Meet Robert Palomino: 'Give Everything a Shot!'

Brookhaven National Laboratory

Tuesday April 22, 2014, 11:30 AM

University of Utah Makes Solar Accessible

University of Utah

Wednesday March 06, 2013, 03:40 PM

Student Innovator at Rensselaer Polytechnic Institute Seeks Brighter, Smarter, and More Efficient LEDs

Rensselaer Polytechnic Institute (RPI)

Friday November 16, 2012, 10:00 AM

Texas Tech Energy Commerce Students, Community Light up Tent City

Texas Tech University

Wednesday November 23, 2011, 10:45 AM

Don't Get 'Frosted' Over Heating Your Home This Winter

Temple University

Wednesday July 06, 2011, 06:00 PM

New Research Center To Tackle Critical Challenges Related to Aircraft Design, Wind Energy, Smart Buildings

Rensselaer Polytechnic Institute (RPI)

Friday April 22, 2011, 09:00 AM

First Polymer Solar-Thermal Device Heats Home, Saves Money

Wake Forest University

Friday April 15, 2011, 12:25 PM

Like Superman, American University Will Get Its Energy from the Sun

American University

Thursday February 10, 2011, 05:00 PM

ARRA Grant to Help Fund Seminary Building Green Roof

University of Chicago

Tuesday December 07, 2010, 05:00 PM

UC San Diego Installing 2.8 Megawatt Fuel Cell to Anchor Energy Innovation Park

University of California San Diego

Monday November 01, 2010, 12:50 PM

Rensselaer Smart Lighting Engineering Research Center Announces First Deployment of New Technology on Campus

Rensselaer Polytechnic Institute (RPI)

Friday September 10, 2010, 12:40 PM

Ithaca College Will Host Regional Clean Energy Summit

Ithaca College

Tuesday July 27, 2010, 10:30 AM

Texas Governor Announces $8.4 Million Award to Create Renewable Energy Institute

Texas Tech University





Showing results

0-4 Of 2215