Assessment of Biomarkers of Subconcussive Head Trauma

Newswise — Charlottesville, VA (May 29, 2018). Researchers from The Sport Science Center at Texas Christian University, Texas Health Sports Medicine, and the University of Wisconsin–La Crosse evaluated the usefulness of biomarker testing in determining the potential extent of brain trauma suffered from repetitive subconcussive head impacts sustained over the course of a college football season. Their findings are reported today in the Journal of Neurosurgery, in the article “Fluctuations in blood biomarkers of head trauma in NCAA football athletes over the course of a season” by Jonathan M. Oliver, Ph.D., and colleagues (https://thejns.org/doi/full/10.3171/2017.12.JNS172035).

In American football, athletes are regularly subjected to subconcussive head impacts—hits to the head that are not severe enough to cause a concussion or even produce a clinical symptom. Nevertheless, the accumulation of subconcussive head impacts over the course of a football season has been linked to neurophysiological and neuropsychological changes in athletes, and the accumulation of subconcussive head impacts over a long career has been suggested as a cause of severe neurodegenerative diseases such as Alzheimer’s disease and chronic traumatic encephalopathy (CTE).

Since individual subconcussive head impacts do not produce symptoms, it can be difficult to ascertain the resultant injury to the brain and to recognize when an athlete should refrain from play. To protect contact-sport athletes throughout the football season, Oliver and colleagues call for the development of a simple, easy-to-use, diagnostic test to identify and monitor accumulation of subconcussive head impacts. The authors suggest periodic measurement of biomarkers of subconcussive head trauma.

In this study, Oliver and colleagues developed a schedule of blood sampling that is cost effective and could fit into the busy schedule of an academic football program. To test the feasibility of this method, they evaluated the usefulness of two well-known biomarkers of head trauma and more severe traumatic brain injury: the tau protein and neurofilament light polypeptide (NF-L).

The researchers obtained blood samples from thirty-five NCAA football players on seven different days, beginning before fall camp commenced and ending three weeks after the competitive football season had finished. The researchers wanted to examine fluctuations in plasma concentrations of tau and serum concentrations of NF-L over the course of the entire football season, during which the number and magnitude of head impacts varied. Those athletes who sustained a concussion were not further examined. Timing of blood sampling was coordinated with the convenience of players and coaches. During the competitive period, this was usually between 36 and 72 hours after a game or practice session.

Twenty football players were identified as starters and fifteen as nonstarters. Since starters are involved in more repetitive plays than nonstarters and are consequently more likely to sustain a greater number of head impacts, the researchers expected to see increased concentrations of biomarkers of brain trauma in starters than in nonstarters, who served as controls in this study.

Analyses of the blood samples showed that tau concentrations decreased over the course of the competitive season, compared to preseason values, in both starters and nonstarters. Concentrations did not increase to preseason values until after the competitive season was over. Statistical analysis showed that differences in tau concentrations between the two groups of athletes (starters and nonstarters) were not great enough to differentiate between starters and nonstarters. In this study, tau concentrations were not useful in determining injury due to repetitive subconcussive head impacts.

Concentrations of NF-L increased in both athlete groups during the competitive season, compared to preseason values. In starters, these increases were significantly higher than preseason values at many time points; in nonstarters, the increases in NF-L concentrations never reached statistical significance. Statistical analysis showed that during periods of repetitive head impacts, differences in NF-L concentrations demonstrated fair to modest accuracy for differentiating between starters and nonstarters.

The researchers conclude that, in this preliminary study, serum NF-L shows some promise as a sensible and reliable biomarker of brain injury due to repetitive subconcussive head impacts. However, they point out that this study is preliminary and that additional, larger studies should be performed to verify this. They believe that the knowledge gained by studies of biomarkers will “lay the groundwork for the eventual development of clinical tools to help reduce the deleterious effects of repetitive subconcussive impacts.” 

When asked about this study, Dr. Oliver responded, “Given recent findings indicating a potential link between repetitive subconcussive impacts and the development of CTE, efforts to determine the effect of subconcussive impacts throughout an athlete’s career may prove useful, especially if those efforts are feasible and cost effective.”


Oliver JM, Anzalone AJ, Stone JD, Turner SM, Blueitt D, Garrison JC, Askow AT, Luedke JE, Jagim AR. Fluctuations in blood biomarkers of head trauma in NCAA football athletes over the course of a season. Journal of Neurosurgery, published online, ahead of print, May 29, 2018; DOI: 10.3171/2017.12.JNS172035.

Drs. Oliver, Anzalone, Stone, Turner, Blueitt, and Garrison are affiliated with The Sport Science Center at Texas Christian University and Texas Health Sports Medicine; Dr. Jagim and Mr. Luedke are affiliated with the University of Wisconsin–La Crosse, Wisconsin; and Mr. Askow is affiliated with The Sport Science Center at Texas Christian University and with the University of Wisconsin–La Crosse, Wisconsin.

Disclosure:  This study was funded in part by a grant from STRUCT Nutrition.




For additional information, please contact: Ms. Jo Ann M. Eliason, Communications Manager, Journal of Neurosurgery Publishing Group, One Morton Drive, Suite 200, Charlottesville, VA 22903. Email: [email protected]; Phone: 434-982-1209.

For 74 years, the Journal of Neurosurgery has been recognized by neurosurgeons and other medical specialists the world over for its authoritative clinical articles, cutting-edge laboratory research papers, renowned case reports, expert technical notes, and more. Each article is rigorously peer reviewed. The Journal of Neurosurgery is published monthly by the JNS Publishing Group, the scholarly journal division of the American Association of Neurological Surgeons. Other peer-reviewed journals published by the JNS Publishing Group each month include Neurosurgical Focus, the Journal of Neurosurgery: Spine, and the Journal of Neurosurgery: Pediatrics. All four journals can be accessed at www.thejns.org.

Founded in 1931 as the Harvey Cushing Society, the American Association of Neurological Surgeons (AANS) is a scientific and educational association with more than 10,000 members worldwide. The AANS is dedicated to advancing the specialty of neurological surgery in order to provide the highest quality of neurosurgical care to the public. All active members of the AANS are certified by the American Board of Neurological Surgery, the Royal College of Physicians and Surgeons (Neurosurgery) of Canada or the Mexican Council of Neurological Surgery, AC. Neurological surgery is the medical specialty concerned with the prevention, diagnosis, treatment and rehabilitation of disorders that affect the entire nervous system including the brain, spinal column, spinal cord, and peripheral nerves. For more information, visit www.AANS.org.


Journal Link: Journal of Neurosurgery, May 29, 2018