FOR RELEASE MARCH 21, 1999
CONTACT: Joshua Sakon, assistant professor, chemistry
(501) 575-7719, [email protected]
Melissa Blouin, science and research communications manager
(501) 575-5555, [email protected]
PHOTO IS AVAILABLE at http://pigtrail.uark.edu/news/art/enzyme.jpg
Caption: The enzyme cellulase clasps cellulose and breaks it into sugar molecules. University of Arkansas chemistry professor Joshua Sakon and his colleagues are the first researchers to speed up this reaction, which may one day lead to mass production of ethanol.
RESEARCHERS FIRST TO DIRECTEDLY INCREASE ENZYME'S ACTIVITY
FAYETTEVILLE, Ark. -- A University of Arkansas chemist has become the first researcher to increase the activity of an enzyme that breaks down one of nature's most abundant materials that can then be converted into ethanol -- a potentially abundant source of environmentally friendly fuel. Joshua Sakon, chemistry, other researchers from the University of Arkansas and scientists from the National Renewable Energy Laboratory in Golden, Colo. study the enzyme cellulase, which cleaves cellulose into glucose, a sugar that can be fermented into ethanol. Intense research has focused on this process because cellulose found in plants accounts for about half the organic material on earth, which makes it potentially a vast renewable energy resource. However, in the reaction that transforms the organic matter into fuel, the low activity of the enzyme cellulase acts as a bottleneck, slowing the whole procedure down. For at least ten years, scientists have sought ways to increase the enzyme's activity, and thus its
productivity -- to no avail. Sakon's group increased the enzyme's activity by 13 percent. The researchers will present their work today (March 21) at the American Chemical Society meeting in Anaheim, Calif. Cellulase cuts the plant tissue cellulose into glucose -- a simple sugar. It does so by attaching to the cellulose, clipping it and releasing the resulting sugar moelcules. "Many people tried to come up with better scissors," Sakon said. He decided to focus instead on the enzyme structures that hold the cellulose in place while it's being clipped. If these structures had a looser grip, he reasoned, they would release the sugars faster, increasing the reaction's activity. The researchers extracted cellulose from fast-growing yellow poplar trees. They used X-ray crystallography to examine the structure of the cellulase-cellulose interaction. Then Sakon worked with University of Arkansas physical chemist Lothar Schaffer, using computer models to predict the impact of modifying the enzyme at certain sites. The researchers then mutated the amino acid tyrosine 245, one of the biochemical "hands" that holds the cellulose in place. They substituted glycine, an amino acid that creates a weaker bond, or grip, at that site.
The resulting mutant increased the enzyme's activity by 13 percent. Sakon already is looking at the next experiment -- perhaps trying mutations elsewhere in the enzyme's "hold-and-release" region. "Our experimental calculations showed we should have got a lot higher activity," Sakon said. "Now something else has become the rate-limiting step."
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