Heart-on-a-Chip: Influence of Static and Perfusion on Cardiac Cells

Article ID: 680140

Released: 21-Sep-2017 2:00 AM EDT

Source Newsroom: SLAS (Society for Laboratory Automation and Screening)

  • Credit: Anna Kobuszewska, Ewelina Tomecka, Kamil Zukowski, Elzbieta Jastrzebska, Michal Chudy, Artur Dybko, Philippe Renaud, Zbigniew Brzozka

    Microsystem with circular chamber

Newswise — A new report in the October 2017 issue of SLAS Technology shows how microsystems can be used to understand processes in heart tissue in detail and to test newly developed compounds applied in the treatment of cardiac diseases. 

Authors from Warsaw University of Technology (Poland) and École Polytechnique Fédérale de Lausanne (Switzerland) explore how the geometry of microsystems and microenvironmental conditions (static and perfusion) influence the proliferation, morphology, and alignment of cardiac cells (rat cardiomyoblasts—H9C2). 

Their results support their hypothesis that external stimulation (continuous medium flow) enhances both the growth and parallel orientation of cardiac (H9C2) cells; and that the use of verapamil, L-type calcium channel blocker, inhibits H9C2 cell proliferation in cultures performed under perfusion conditions. The authors observe that these parameters depend on the geometry of the culture microchamber.

To study cell growth after incubation with verapamil hydrochloride, poly(dimethylsiloxane) (PDMS)/glass microfluidic systems with three different geometries of microchambers (a circular chamber, a longitudinal channel, and three parallel microchannels separated by two rows of micropillars) are prepared. The authors find that static conditions do not enhance the growth of H9C2 cells in the microsystems. On the contrary, perfusion conditions have an influence on division, morphology, and the arrangement of the cells. The highest number of cells, their parallel orientation, and their elongated morphology are obtained in the longitudinal microchannel, showing that this kind of microsystem can be used to understand processes in heart tissue in detail and to test newly developed compounds applied in the treatment of cardiac diseases.

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Visit SLAS Technology Online to read "Heart-on-a-Chip: Influence of Static and Perfusion on Cardiac Cell Proliferation, Morphology, and Alignment." SLAS Technology is one of two MEDLINE-indexed scientific journals published by SLAS. For more information about SLAS and its journals, visit www.slas.org/journals.

About our Society and Journals

SLAS (Society for Laboratory Automation and Screening) is an international community of nearly 20,000 professionals and students dedicated to life sciences discovery and technology. The SLAS mission is to bring together researchers in academia, industry and government to advance life sciences discovery and technology via education, knowledge exchange and global community building. 

SLAS DISCOVERY: 2016 Impact Factor 2.444. Editor-in-Chief Robert M. Campbell, Ph.D., Eli Lilly and Company, Indianapolis, IN (USA). SLAS Discovery (Advancing Life Sciences R&D) was previously published (1996-2016) as the Journal of Biomolecular Screening (JBS).

SLAS TECHNOLOGY: 2016 Impact Factor 2.850. Editor-in-Chief Edward Kai-Hua Chow, Ph.D., National University of Singapore (Singapore). SLAS Technology (Translating Life Sciences Innovation) was previously published (1996-2016) as the Journal of Laboratory Automation (JALA).

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