Growth status and indicators of CR lines of L. bicolor. a Growth status of transgenic plants growing in 200 mM NaCl medium at 0, 10, and 20 days. Scale bar = 5 mm. b Leaf lethality rates of transgenic lines. Data are means ± standard deviation of nine replicates. c Staining of leaves of regenerated lines. Scale bar = 1 mm. d Statistics of the degree of staining. Data are means ± standard deviation of nine replicates. SPSS was used to determine the statistical significance of the data in the t-test. ***P < 0.001. e Sequence alignment of Lb1G07934 from the CRISPR mutants CR-2 and CR-4, showing DNA alignments. Red lines indicate the CRISPR target sites; yellow boxes indicate the PAM sequence. f Appearance comparison of three lines before and after treatment with 200 mM NaCl. Scale bar = 5 cm. g Na+, K+, MDA, and proline contents in three lines before and after 200 mM NaCl treatment. Data are means ± standard deviation of three replicates. SPSS was used to determine the statistical significance of the data. Different letters indicate significant differences (p = 0.05; Duncan’s multiple range test).