Phenotypic analyses of the brfls1 T2 plants. (A) Cross-sections of heads of two brfls1 T2 plants and controls (8045 and NB61–99). (B) Indices related to agricultural traits of their heads (C) Root lengths of seedlings of brfls1 T2 plants and control plants four (for control treatment) or six (for mannitol or NaCl treatment) days after osmolytes treatment. (D) Chlorophyll contents measured from aerial parts of the seedlings 4 days after mannitol treatment. The mean values ± SD of four independent biological samples are shown. Statistical significance was determined by Duncan’s Multiple Range Test using SAS (version 9.1) software. Significant differences between means (P < 0.05) are indicated by different lower case letters (a, b, and c).