Newswise — Scientists from Queen Mary University of London and UCL have shown that fetal membranes are able to heal after injury in a new study published today in Scientific Reports.

The integrity of the fetal membranes that surround the baby in the womb during pregnancy is vital for normal development. But fetal membranes can become damaged as a result of infection, bleeding, or after fetal surgery and even diagnostic tests during pregnancy, such as amniocentesis, which require doctors to make a hole with a needle in the fetal membrane sac.

Currently there are no clinical approaches available to repair or improve healing in the fetal membranes, and until now it was unclear if small holes in the membranes were able to heal themselves.

The international research team, which also includes scientists and clinicians from Nanyang Technological University, Singapore and University Hospitals Leuven, Belgium, created small defects using a needle in donated human fetal membrane tissue, to mimic damage caused during fetal surgery. A few days after injury, the researchers discovered a population of cells called myofibroblasts (MFs), which play an important role in wound healing, and found that these cells crawled towards the edges of the wound and into the defect site. This cell population produced collagen and started to pull the edges of the wound, contracting the tissues together and repairing the wound.   

The findings follow on from the team’s previous work that highlighted the importance of a protein called Connexin 43 (Cx43) in the process of wound healing and repair. Whilst in this study, the researchers show that Cx43 was expressed by two cell populations, amniotic mesenchymal cells (AMCs) and MFs, the localisation and levels of Cx43 measured were different. They also found that overexpression of this protein affected the ability of cells to migrate into the defect site and close the wound.

Dr Tina Chowdhury, Senior Lecturer in Regenerative Medicine at Queen Mary, said: “We have always thought that small diameter wounds created in human fetal membranes rarely heal by themselves but here we show that the tissues have the potential to do this. We found that Cx43 has different effects on cell populations found in the membranes and promotes transformation of AMCs into MFs, triggering them to move, repair and heal defects in the fetal membranes.”

The premature rupture of fetal membranes, known as preterm prelabour rupture of the membranes (PPROM), is a major cause of preterm birth accounting for around 40 per cent of early infant death. Therefore, the successful repair of fetal membranes could help reduce the risk of birth complications.

Anna David, UCLH Consultant and Professor in Obstetrics and Maternal Fetal Medicine and Director at the UCL Elizabeth Garrett Anderson Institute for Women’s Health and a co-author of the study, said: “Finding that the fetal membranes have this potential to heal is a huge step towards developing treatments for women with PPROM. It holds out hope that we may be able to delay or even prevent preterm birth, which will significantly improve baby outcomes.”

This research was funded by Great Ormond Street Hospital Children’s Charity (17QMU01), Rosetrees Trust (M808), KU Leuven University Fund, Little Heartbeats (https://www.little-heartbeats.org.uk/) and the Prenatal Therapy Fund, University College London Hospital Charity (https://www.justgiving.com/fundraising/uclh-prenatal-therapy-fund).

Notes to editors

  • Research publication: ‘Cx43 mediates changes in myofibroblast contraction and collagen release in human amniotic membrane defects after trauma’ Eleni Costa, Babatunde O. Okesola, Christopher Thrasivoulou, David L. Becker, Jan A. Deprest, Anna L. David, Tina T. Chowdhury, Scientific Reports.

 

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Queen Mary University of London is a research-intensive university that connects minds worldwide. A member of the prestigious Russell Group, we work across the humanities and social sciences, medicine and dentistry, and science and engineering, with inspirational teaching directly informed by our world-leading research. In the most recent Research Excellence Framework we were ranked 5th in the country for the proportion of research outputs that were world-leading or internationally excellent. We have over 25,000 students and offer more than 240 degree programmes. Our reputation for excellent teaching was rewarded with silver in the most recent Teaching Excellence Framework. Queen Mary has a proud and distinctive history built on four historic institutions stretching back to 1785 and beyond. Common to each of these institutions – the London Hospital Medical College, St Bartholomew’s Medical College, Westfield College and Queen Mary College – was the vision to provide hope and opportunity for the less privileged or otherwise under-represented. Today, Queen Mary University of London remains true to that belief in opening the doors of opportunity for anyone with the potential to succeed and helping to build a future we can all be proud of.

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