DOE News
    Doe Science news source
    The DOE Science News Source is a Newswise initiative to promote research news from the Office of Science of the DOE to the public and news media.
    • 2016-09-07 05:00:57
    • Article ID: 660328

    How Fungi Help Trees Tolerate Drought

    Genome of world's most common fungal symbiont sheds light on drought resistance role

    • Credit: Maira de Freitas Pereira, INRA Nancy

      The crosscut shows the fungal tissues - comprised of he fungal mantle around the root tip and the fungal network of tendrils that penetrates the root of plants, or Hartig Net, between Pinus sylvestris plant root cells - in green.

    • Credit: Martina Peter, Swiss Federal Research Institute WSL

      These are poplar roots and the black mycelium and mycorrhizas formed by the Cenococcum geophilum fungus.

    The mutualistic relationship between tree roots and ectomycorrhizal (ECM) fungi has been shaping forest ecosystems since their inception. ECM fungi are key players supporting the growth, health and stress tolerance of forest trees globally, such as oak, pine, spruce, birch and beech, and help boost the productivity of bioenergy feedstock trees, including poplar and willow. The most common ECM fungus is Cenococcum geophilum, found in subtropical through arctic zones and especially in extreme environments. It is also the only mycorrhizal fungus in the Dothideomycetes, a large class comprised of some 19,000 fungal species, many of them plant pathogens.

    To learn more about what ectomycorrhizal characteristics are dominant in Cenococcum geophilum, a team led by researchers at the French National Institute for Agricultural Research (INRA) and the Swiss Federal Institute for Forest, Snow and Landscape Research WSL, and including researchers at the U.S. Department of Energy Joint Genome Institute (DOE JGI), a DOE Office of Science User Facility, compared its genome with the genomes of close relatives Lepidopterella palustris and Glonium stellatum, neither of which are ECM fungi. The study was published online September 7 in Nature Communications. They found specific adaptations in the C. geophilum transcriptome ¬– the set of its messenger RNA molecules that reflects actual biochemical activity by the fungus ¬–that could help their hosts be more resistant to drought stress, a finding that could be useful in developing more plant feedstocks for bioenergy amidst the changing climate.

    As part of a comparative genomic analysis done through the Mycorrhizal Genomics Initiative (MGI) headed by study senior author Francis Martin of INRA, the DOE JGI sequenced C. geophilum and its close relative Lepidopterella palustris, and annotated both of these genomes and another close relative, Glonium stellatum. “We showed that the genome of C. geophilum, the only known mycorrhizal symbiont within the largest fungal class Dothideomycetes, acquired the same genomic adaptations to the mycorrhizal lifestyle over generations as the previously sequenced ectomycorrhizal basidiomycetes,” Martin said. “These include a strikingly reduced number of plant cell wall degrading enzymes (PCWDEs) and a large set of symbiosis-induced lineage-specific genes, including dozen of mycorrhiza-induced small secreted effector-like proteins (MiSSPs).”Unlike free-living saprotrophs, fungi that get their nutrients from decomposing organic matter in forest soils and so require PCWDEs, Cenoccocum has come to rely heavily on its hosts for its carbon nutrition.

    Key Genes for Drought Adaptation Found

    Noting that the root tips of C. geophilum are highly resistant to dessication, one of the team’s key findings is that two of the three most highly induced C. geophilum genes in symbiosis code for water channels. “The regulation of these water channel genes is fine-tuned under drought conditions and they might therefore play a key role in drought adaptation of host plants,” said first author Martina Peter of the Swiss Federal Research Institute WSL.

    C. geophilum population genomics should shed light on the mechanisms of host and environmental adaptation,” the team wrote in their paper. “It should facilitate the identification of drought-adapted C. geophilum strains, which can be used to efficiently support their host trees threatened by the forecasted increase in drought periods in many parts of the world.”

    Both Peter and Martin noted that credit for the success of the work and of the Mycorrhizal Genomics Initiative goes to the “tight collaboration” between their teams, the DOE JGI, Joey Spatafora at Oregon State University and Pedro Crous CBS Fungal Biodiversity Centre (Utrecht, Netherlands) as well as with Bernard Henrissat of CNRS and University of Aix-Marseilles.

    “The intersection of genomics and evolutionary biology, as carried out in the MGI and the 1000 Fungal Genomes (KFG) Project, can inform our understanding of the biological principles intrinsic to mycorrhizal symbiosis,” said Martin. “By combining genome sequences with rigorous physiological and ecological studies, we are entering a time where linking the presence, composition and abundance of soil mycorrhizal communities with important soil processes and forest productivity at an ecosystem scale is possible.”

    ***

    The U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility at Lawrence Berkeley National Laboratory, is committed to advancing genomics in support of DOE missions related to clean energy generation and environmental characterization and cleanup. DOE JGI, headquartered in Walnut Creek, Calif., provides integrated high-throughput sequencing and computational analysis that enable systems-based scientific approaches to these challenges. Follow @doe_jgi on Twitter.

    DOE’s Office of Science is the largest supporter of basic research in the physical sciences in the United States, and is working to address some of the most pressing challenges of our time. For more information, please visit science.energy.gov.

    • other-fb
    • other-tw
    X
    X
    X
    • Filters

    • × Clear Filters
    Blowing bubbles: PPPL scientist confirms novel way to launch and drive current in fusion plasmas

    Blowing bubbles: PPPL scientist confirms novel way to launch and drive current in fusion plasmas

    PPPL physicist Fatima Ebrahimi has used high-resolution computer simulations to confirm the practicality of the CHI start-up technique. The simulations show that CHI could produce electric current continuously in larger, more powerful tokamaks than exist today to produce stable fusion plasmas.

    A four-way switch promises greater tunability of layered materials

    A four-way switch promises greater tunability of layered materials

    A team from Oak Ridge National Laboratory and Vanderbilt University made the first experimental observation of a material phase that had been predicted but never seen.

    Scientists discover ​"ripple" in flexible material that could improve electronic properties

    Scientists discover ​"ripple" in flexible material that could improve electronic properties

    Argonne scientists have discovered an intriguing new behavior in a two-dimensional material at the atomic level as it is stretched and strained, like it would be in an actual flexible device.

    AI for Plant Breeding in an Ever-Changing Climate

    AI for Plant Breeding in an Ever-Changing Climate

    In this Q&A, Oak Ridge National Laboratory's Dan Jacobson talks about his team's work on a genomic selection algorithm, his vision for the future of environmental genomics, and the space where simulation meets AI.

    A New Parallel Strategy for Tackling Turbulence on Summit

    A New Parallel Strategy for Tackling Turbulence on Summit

    A team at Georgia Tech created a new turbulence algorithm optimized for the Summit supercomputer. It reached a performance of less than 15 seconds of wall-clock time per time step for more than 6 trillion grid points--a new world record surpassing the prior state of the art in the field for the size of the problem.

    Modeling Every Building in America Starts with Chattanooga

    Modeling Every Building in America Starts with Chattanooga

    An ORNL team used the Titan supercomputer to model every building serviced by the Electric Power Board of Chattanooga--all 178,368 of them--and discovered that EPB could potentially save $11-$35 million per year by adjusting electricity usage during peak critical times.

    Climate Change Expected to Shift Location of East Asian Monsoons

    Climate Change Expected to Shift Location of East Asian Monsoons

    More than a billion people in Asia depend on seasonal monsoons for their water needs. The Asian monsoon is closely linked to a planetary-scale tropical air flow which, according to a new study by Lawrence Berkeley National Laboratory, will most likely shift geographically as the climate continues to warm, resulting in less rainfall in certain regions.

    Nuclear warheads? This robot can find them

    Nuclear warheads? This robot can find them

    PPPL and Princeton University are developing a unique neutron-detector robot for arms control and nuclear security purposes. The robot recently passed a key neutron-detection test.

    Deep neural networks speed up weather and climate models

    Deep neural networks speed up weather and climate models

    A team of environmental and computation scientists at the U.S. Department of Energy's (DOE) Argonne National Laboratory are collaborating to use deep neural networks, a type of machine learning, to replace the parameterizations of certain physical schemes in the Weather Research and Forecasting Model, an extremely comprehensive model that simulates the evolution of many aspects of the physical world around us.

    New AI Model Tries to Synthesize Patient Data Like Doctors Do

    New AI Model Tries to Synthesize Patient Data Like Doctors Do

    A new approach developed by PNNL scientists improves the accuracy of patient diagnosis up to 20 percent when compared to other embedding approaches.


    • Filters

    • × Clear Filters
    University of Maryland, Baltimore County wins DOE's 2019 CyberForce Competition(tm)

    University of Maryland, Baltimore County wins DOE's 2019 CyberForce Competition(tm)

    After a long suspenseful day, University of Maryland, Baltimore County earned the top spot as national winner of the U.S. Department of Energy's CyberForce Competition.

    In its 15th year, INCITE advances open science with supercomputer grants to 47 projects

    In its 15th year, INCITE advances open science with supercomputer grants to 47 projects

    The U.S. Department of Energy's Office of Science announced allocations of supercomputer access to 47 science projects for 2020--awarding 60 percent of the available time on some of the nation's most powerful supercomputers, with the ultimate goal of accelerating discovery and innovation. In 2020, 14 projects will run on Theta and 39 projects on Summit, where six of these projects will receive an allocation on both systems.

    ASU solar awards eclipse other universities in latest round of DOE funding

    ASU solar awards eclipse other universities in latest round of DOE funding

    ASU receives $9.8 million in Solar Energy Technologies Office Awards.

    DOE to Provide $10 Million for New Research into Ecosystem Processes

    The U.S. Department of Energy (DOE) announced a plan to provide $10 million for new observational and experimental studies aimed at improving the accuracy of today's Earth system models. Research will focus on three separate types of environments--terrestrial, watershed, and subsurface--where current models fall short of providing fully accurate representation.

    ORNL to host 13 teams for DOE CyberForce Competition

    ORNL to host 13 teams for DOE CyberForce Competition

    Oak Ridge National Laboratory will give college students the chance to practice cybersecurity skills in a real-world setting as a host of the Department of Energy's fifth collegiate CyberForce Competition on Nov. 16.

    Argonne nuclear engineer J'Tia Hart selected to Crain's Chicago Business "40 Under 40"

    Argonne nuclear engineer J'Tia Hart selected to Crain's Chicago Business "40 Under 40"

    Argonne nuclear engineer J'Tia Hart has been named to Crain's Chicago Business's "40 Under 40" list, which recognizes young leaders in a variety of fields.

    Lab-Wide Stormwater Capture, Transportation Savings and Clean-Up Efforts Win Federal Recognition

    Lab-Wide Stormwater Capture, Transportation Savings and Clean-Up Efforts Win Federal Recognition

    Argonne National Laboratory has won a regional Federal Green Challenge award for conserving resources and saving taxpayers' money.

    PPPL wins $70,000 in project funding from DOE for entrepreneurship

    PPPL wins $70,000 in project funding from DOE for entrepreneurship

    The Princeton Plasma Physics Laboratory receives funding from the U.S. Department of Energy for two projects to encourage entrepreneurship and mentor and encourage potential entrepreneurs.

    Brookhaven-Commonwealth Fusion Energy Project Wins DOE Funding

    Brookhaven-Commonwealth Fusion Energy Project Wins DOE Funding

    Brookhaven's Superconducting Magnet Division will partner with industry to develop and characterize superconducting power cables.

    U.S. Department of Energy to Hold Fifth CyberForce Competition(tm)

    U.S. Department of Energy to Hold Fifth CyberForce Competition(tm)

    Going on its fourth year, DOE's CyberForce Competition(tm) on Nov. 15-16 will give teams of cybersecurity students and professionals the opportunity to compete and refine their skills in real-time at 10 national laboratories across the U.S.


    • Filters

    • × Clear Filters
    Harvesting Energy from Light using Bio-inspired Artificial Cells

    Harvesting Energy from Light using Bio-inspired Artificial Cells

    Scientists designed and connected two different artificial cells to each other to produce molecules called ATP (adenosine triphosphate).

    Engineering Living Scaffolds for Building Materials

    Engineering Living Scaffolds for Building Materials

    Bone and mollusk shells are composite systems that combine living cells and inorganic components. This allows them to regenerate and change structure while also being very strong and durable. Borrowing from this amazing complexity, researchers have been exploring a new class of materials called engineered living materials (ELMs).

    Excavating Quantum Information Buried in Noise

    Excavating Quantum Information Buried in Noise

    Researchers developed two new methods to assess and remove error in how scientists measure quantum systems. By reducing quantum "noise" - uncertainty inherent to quantum processes - these new methods improve accuracy and precision.

    How Electrons Move in a Catastrophe

    How Electrons Move in a Catastrophe

    Lanthanum strontium manganite (LSMO) is a widely applicable material, from magnetic tunnel junctions to solid oxide fuel cells. However, when it gets thin, its behavior changes for the worse. The reason why was not known. Now, using two theoretical methods, a team determined what happens.

    When Ions and Molecules Cluster

    When Ions and Molecules Cluster

    How an ion behaves when isolated within an analytical instrument can differ from how it behaves in the environment. Now, Xue-Bin Wang at Pacific Northwest National Laboratory devised a way to bring ions and molecules together in clusters to better discover their properties and predict their behavior.

    Tune in to Tetrahedral Superstructures

    Tune in to Tetrahedral Superstructures

    Shape affects how the particles fit together and, in turn, the resulting material. For the first time, a team observed the self-assembly of nanoparticles with tetrahedral shapes.

    Tracing Interstellar Dust Back to the Solar System's Formation

    Tracing Interstellar Dust Back to the Solar System's Formation

    This study is the first to confirm dust particles pre-dating the formation of our solar system. Further study of these materials will enable a deeper understanding of the processes that formed and have since altered them.

    Investigating Materials that Can Go the Distance in Fusion Reactors

    Investigating Materials that Can Go the Distance in Fusion Reactors

    Future fusion reactors will require materials that can withstand extreme operating conditions, including being bombarded by high-energy neutrons at high temperatures. Scientists recently irradiated titanium diboride (TiB2) in the High Flux Isotope Reactor (HFIR) to better understand the effects of fusion neutrons on performance.

    Better 3-D Imaging of Tumors in the Breast with Less Radiation

    Better 3-D Imaging of Tumors in the Breast with Less Radiation

    In breast cancer screening, an imaging technique based on nuclear medicine is currently being used as a successful secondary screening tool alongside mammography to improve the accuracy of the diagnosis. Now, a team is hoping to improve this imaging technique.

    Microbes are Metabolic Specialists

    Microbes are Metabolic Specialists

    Scientists can use genetic information to measure if microbes in the environment can perform specific ecological roles. Researchers recently analyzed the genomes of over 6,000 microbial species.


    Spotlight

    Barbara Garcia: A first-generation college student spends summer doing research at PPPL
    Tuesday September 24, 2019, 04:05 PM

    Barbara Garcia: A first-generation college student spends summer doing research at PPPL

    Princeton Plasma Physics Laboratory

    Argonne organization's scholarship fund blazes STEM pathway
    Tuesday September 17, 2019, 05:05 PM

    Argonne organization's scholarship fund blazes STEM pathway

    Argonne National Laboratory

    Brookhaven Lab, Suffolk Girl Scouts Launch Patch Program
    Friday September 13, 2019, 11:30 AM

    Brookhaven Lab, Suffolk Girl Scouts Launch Patch Program

    Brookhaven National Laboratory

    From an acoustic levitator to a
    Thursday September 12, 2019, 03:05 PM

    From an acoustic levitator to a "Neutron Bloodhound" robot, hands-on research inspires PPPL's summer interns

    Princeton Plasma Physics Laboratory

    Brookhaven Lab Celebrates the Bright Future of its 2019 Interns
    Friday August 30, 2019, 10:00 AM

    Brookhaven Lab Celebrates the Bright Future of its 2019 Interns

    Brookhaven National Laboratory

    PPPL apprenticeship program offers young people chance to earn while they learn high-tech careers
    Thursday August 01, 2019, 12:05 PM

    PPPL apprenticeship program offers young people chance to earn while they learn high-tech careers

    Princeton Plasma Physics Laboratory

    Creating a diverse pipeline
    Friday July 19, 2019, 01:05 PM

    Creating a diverse pipeline

    Princeton Plasma Physics Laboratory

    JSA Awards Graduate Fellowships for Research at Jefferson Lab
    Monday July 08, 2019, 03:00 PM

    JSA Awards Graduate Fellowships for Research at Jefferson Lab

    Thomas Jefferson National Accelerator Facility

    ILSAMP Symposium showcases benefits for diverse students, STEM pipeline
    Monday May 20, 2019, 12:05 PM

    ILSAMP Symposium showcases benefits for diverse students, STEM pipeline

    Argonne National Laboratory

    Integrating Scientific Computing into Science Curricula
    Monday May 13, 2019, 11:05 AM

    Integrating Scientific Computing into Science Curricula

    Brookhaven National Laboratory

    Students from Minnesota and Massachusetts Win DOE's 29th National Science Bowl(r)
    Monday April 29, 2019, 02:05 PM

    Students from Minnesota and Massachusetts Win DOE's 29th National Science Bowl(r)

    Department of Energy, Office of Science

    DOE's Science Graduate Student Research Program Selects 70 Students to Pursue Research at DOE Laboratories
    Friday April 12, 2019, 03:05 PM

    DOE's Science Graduate Student Research Program Selects 70 Students to Pursue Research at DOE Laboratories

    Department of Energy, Office of Science

    Young Women's Conference in STEM seeks to change the statistics one girl at a time
    Thursday March 28, 2019, 03:05 PM

    Young Women's Conference in STEM seeks to change the statistics one girl at a time

    Princeton Plasma Physics Laboratory

    Students team with Argonne scientists and engineers to learn about STEM careers
    Tuesday March 12, 2019, 05:05 PM

    Students team with Argonne scientists and engineers to learn about STEM careers

    Argonne National Laboratory

    Lynbrook High wins 2019 SLAC Regional Science Bowl competition
    Wednesday February 13, 2019, 02:05 PM

    Lynbrook High wins 2019 SLAC Regional Science Bowl competition

    SLAC National Accelerator Laboratory

    Equipping the next generation for a technological revolution
    Thursday January 24, 2019, 01:05 PM

    Equipping the next generation for a technological revolution

    Argonne National Laboratory

    Chemistry intern inspired by Argonne's real-world science
    Friday January 18, 2019, 05:05 PM

    Chemistry intern inspired by Argonne's real-world science

    Argonne National Laboratory

    Chasing a supernova
    Friday January 18, 2019, 04:05 PM

    Chasing a supernova

    Argonne National Laboratory

    Argonne intern streamlines the beamline
    Tuesday January 08, 2019, 02:05 PM

    Argonne intern streamlines the beamline

    Argonne National Laboratory

    Research on Light-Matter Interaction Could Lead to Improved Electronic and Optoelectronic Devices
    Thursday October 11, 2018, 04:00 PM

    Research on Light-Matter Interaction Could Lead to Improved Electronic and Optoelectronic Devices

    Rensselaer Polytechnic Institute (RPI)

    Innovating Our Energy Future
    Wednesday October 03, 2018, 07:05 PM

    Innovating Our Energy Future

    Oregon State University, College of Engineering

    Physics graduate student takes her thesis research to a Department of Energy national lab
    Tuesday October 02, 2018, 03:05 PM

    Physics graduate student takes her thesis research to a Department of Energy national lab

    University of Alabama at Birmingham

    Friday September 21, 2018, 01:05 PM

    "Model" students enjoy Argonne campus life

    Argonne National Laboratory

    Writing Code for a More Skilled and Diverse STEM Workforce
    Thursday September 06, 2018, 01:05 PM

    Writing Code for a More Skilled and Diverse STEM Workforce

    Brookhaven National Laboratory

    New graduate student summer school launches at Princeton Plasma Physics Laboratory
    Tuesday September 04, 2018, 11:30 AM

    New graduate student summer school launches at Princeton Plasma Physics Laboratory

    Princeton Plasma Physics Laboratory

    The Gridlock State
    Friday August 31, 2018, 06:05 PM

    The Gridlock State

    California State University (CSU) Chancellor's Office

    Meet Jasmine Hatcher and Trishelle Copeland-Johnson
    Friday August 31, 2018, 02:05 PM

    Meet Jasmine Hatcher and Trishelle Copeland-Johnson

    Brookhaven National Laboratory

    Argonne hosts Modeling, Experimentation and Validation Summer School
    Friday August 24, 2018, 11:05 AM

    Argonne hosts Modeling, Experimentation and Validation Summer School

    Argonne National Laboratory

    Students affected by Hurricane Maria bring their research to SLAC
    Wednesday August 22, 2018, 01:05 PM

    Students affected by Hurricane Maria bring their research to SLAC

    SLAC National Accelerator Laboratory

    Brookhaven Lab Pays Tribute to 2018 Summer Interns
    Wednesday August 22, 2018, 10:05 AM

    Brookhaven Lab Pays Tribute to 2018 Summer Interns

    Brookhaven National Laboratory

    Changing How Buildings Are Made
    Monday August 20, 2018, 12:05 PM

    Changing How Buildings Are Made

    Washington University in St. Louis

    CSUMB Selected to Host Architecture at Zero Competition in 2019
    Thursday August 16, 2018, 12:05 PM

    CSUMB Selected to Host Architecture at Zero Competition in 2019

    California State University, Monterey Bay

    Department of Energy Invests $64 Million in Advanced Nuclear Technology
    Friday July 20, 2018, 03:00 PM

    Department of Energy Invests $64 Million in Advanced Nuclear Technology

    Rensselaer Polytechnic Institute (RPI)

    Professor Miao Yu Named the Priti and Mukesh Chatter '82 Career Development Professor
    Thursday July 19, 2018, 05:00 PM

    Professor Miao Yu Named the Priti and Mukesh Chatter '82 Career Development Professor

    Rensselaer Polytechnic Institute (RPI)

    2018 RHIC & AGS Annual Users' Meeting: 'Illuminating the QCD Landscape'
    Tuesday July 03, 2018, 11:05 AM

    2018 RHIC & AGS Annual Users' Meeting: 'Illuminating the QCD Landscape'

    Brookhaven National Laboratory

    Argonne welcomes <em>The Martian</em> author Andy Weir
    Friday June 29, 2018, 06:05 PM

    Argonne welcomes The Martian author Andy Weir

    Argonne National Laboratory

    Creating STEM Knowledge and Innovations to Solve Global Issues Like Water, Food, and Energy
    Monday June 18, 2018, 09:55 AM

    Creating STEM Knowledge and Innovations to Solve Global Issues Like Water, Food, and Energy

    Illinois Mathematics and Science Academy (IMSA)

    Professor Emily Liu Receives $1.8 Million DoE Award for Solar Power Systems Research
    Friday June 15, 2018, 10:00 AM

    Professor Emily Liu Receives $1.8 Million DoE Award for Solar Power Systems Research

    Rensselaer Polytechnic Institute (RPI)

    Celebrating 40 years of empowerment in science
    Thursday June 07, 2018, 03:05 PM

    Celebrating 40 years of empowerment in science

    Argonne National Laboratory

    Introducing Graduate Students Across the Globe to Photon Science
    Monday May 07, 2018, 10:30 AM

    Introducing Graduate Students Across the Globe to Photon Science

    Brookhaven National Laboratory

    Students from Massachusetts and Washington Win DOE's 28th National Science Bowl(r)
    Wednesday May 02, 2018, 04:05 PM

    Students from Massachusetts and Washington Win DOE's 28th National Science Bowl(r)

    Department of Energy, Office of Science

    The Race for Young Scientific Minds
    Thursday April 12, 2018, 07:05 PM

    The Race for Young Scientific Minds

    Argonne National Laboratory

    Q&A: Al Ashley Reflects on His Efforts to Diversify SLAC and Beyond
    Wednesday March 14, 2018, 02:05 PM

    Q&A: Al Ashley Reflects on His Efforts to Diversify SLAC and Beyond

    SLAC National Accelerator Laboratory

    Insights on Innovation in Energy, Humanitarian Aid Highlight UVA Darden's Net Impact Week
    Thursday February 15, 2018, 12:05 PM

    Insights on Innovation in Energy, Humanitarian Aid Highlight UVA Darden's Net Impact Week

    University of Virginia Darden School of Business

    Ivy League Graduate, Writer and Activist with Dyslexia Visits CSUCI to Reframe the Concept of Learning Disabilities
    Friday February 09, 2018, 11:05 AM

    Ivy League Graduate, Writer and Activist with Dyslexia Visits CSUCI to Reframe the Concept of Learning Disabilities

    California State University, Channel Islands

    Photographer Adam Nadel Selected as Fermilab's New Artist-in-Residence for 2018
    Wednesday January 17, 2018, 12:05 PM

    Photographer Adam Nadel Selected as Fermilab's New Artist-in-Residence for 2018

    Fermi National Accelerator Laboratory (Fermilab)





    Showing results

    0-4 Of 2215