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The DOE Science News Source is a Newswise initiative to promote research news from the Office of Science of the DOE to the public and news media.
  • 2018-03-07 14:05:54
  • Article ID: 690742

With Laser Light, Scientists Create First X-Ray Holographic Images of Viruses

Using SLAC's X-ray laser, researchers have made the detailed 3-D images of nanoscale biology, with future applications in the study of air pollution, combustion and catalytic processes.

  • Credit: Anatoli Ulmer and Taisia Gorkhover / The Technical University of Berlin and SLAC National Accelerator Laboratory

    This image shows simulated X-ray diffraction patterns that form an in-flight hologram. The depth information is captured from the curved lines.

  • Credit: Anatoli Ulmer and Taisia Gorkhover / The Technical University of Berlin and SLAC National Accelerator Laboratory

    In the new study, the authors superimposed scattered X-ray light from the mimivirus with scattered X-ray light from a reference sphere (main image). The curvature in the superimposed images from the two objects provided depth information and details about the shape of the virus. The image in the lower right corner is a holographic reconstruction of the virus based on the X-ray diffraction patterns collected during the experiment.

  • Credit: Anatoli Ulmer and Taisia Gorkhover / The Technical University of Berlin and SLAC National Accelerator Laboratory

    Illustration showing the principle of in-flight holography. (Left) The X-rays scatter off two spheres and form a characteristic diffraction pattern. The patterns are recorded using the very intense X-ray beam of SLAC’s X-ray laser, the Linac Coherent Light Source (LCLS). (Center) Changes in size and distance of the spheres are reflected in the patterns which can be directly translated from the diffraction alone. The smaller sphere can act as a holographic reference. (Right) If the spheres are shifted out of plane, the fine lines of the diffraction pattern become curved. The signatures of the position and size of the reference allow researchers to reconstruct the 3-D distances between the small sphere (reference) and the large sphere.

Holography, like photography, is a way to record the world around us. Both use light to make recordings, but instead of two-dimensional photos, holograms reproduce three-dimensional shapes. The shape is inferred from the patterns that form after light ricochets off an object and interferes with another light wave that serves as a reference.

When created with X-ray light, holography can be an extremely useful method for capturing high-resolution images of a nanoscale object—something that is so small, its size is measured in nanometers, or billionths of a meter.

So far, X-ray holography has been restricted to objects that form crystals or relied on careful positioning of the sample on a surface. However, many nano-sized particles are non-crystalline, short-lived and very fragile. They may also suffer changes or damage during an experiment when positioned on a surface. Aerosols, exotic states of matter, and the smallest forms of life often fall into these categories and therefore are difficult to study with conventional imaging methods.

In a recent study featured on the March 2018 cover of Nature Photonics, researchers developed a new holographic method called in-flight holography. With this method, they were able to demonstrate the first X-ray holograms of nano-sized viruses that were not attached to any surface.

The patterns needed to create the images were taken at the Linac Coherent Light Source (LCLS), the X-ray free-electron laser at the Department of Energy’s SLAC National Accelerator Laboratory. Nanoviruses have been studied at LCLS without a holographic reference, but the interpretation of the X-ray images required many steps, relied on human input and was a computationally challenging task.

In the new study, the authors superimposed scattered X-ray light from the virus with scattered X-ray light from a reference nano-sized sphere. The curvature in the superimposed images from the two objects provided depth information and details about the shape of the 450-nanometer-wide virus, the mimivirus. This technique greatly simplified the interpretation of the data.

“Instead of thousands of steps and algorithms that potentially don’t match up, you have a two-step procedure where you clearly get the structure out of your image,” says lead study author Tais Gorkhover, a Panofsky Fellowat SLAC and researcher at the Stanford PULSE Institute.

Now, the scientists can do their reconstruction of a sample in fractions of a second or even faster with the holographic method.

“Before our study, the interpretation of the X-ray images was very complicated and the structure of nanosamples was reconstructed long after the actual experiment using non-trivial algorithms,” says Christoph Bostedt, a scientist at the DOE’s Argonne National Laboratory and a co-author of the study. “With ‘in-flight’ holography, the procedure is very simple and in principle can be performed while taking data. This is a real breakthrough.”

“Another advantage of the in-flight holography method is that it is less prone to noise and to the artifacts that can appear in the detector compared to non-holographic X-ray imaging,” says Anatoli Ulmer, a co-author and PhD student from the Technical University of Berlin in Germany.

In the long run, the researchers predict that in-flight holography will offer new ways to study air pollution, combustion and catalytic processes, all of which involve nanoparticles.

An international team of scientists from the DOE’s Lawrence Berkeley and Brookhaven national laboratories, the Chalmers University of Technology in Sweden, the Czech Academy of Science, Deutsches Elektronen-Synchrotron (DESY) in Germany, the Institute of Optics and Atomic Physics in Germany, the Institute for Solid State Physics and Optics in Hungary, KTH Royal Institute of Technology in Sweden, Northwestern University and Uppsala University in Sweden also contributed to this research.

LCLS is a DOE Office of Science user facility.

SLAC is a multi-program laboratory exploring frontier questions in photon science, astrophysics, particle physics and accelerator research. Located in Menlo Park, Calif., SLAC is operated by Stanford University for the U.S. Department of Energy's Office of Science. For more informatiom, please visit slac.stanford.edu.

SLAC National Accelerator Laboratory is supported by the Office of Science of the U.S. Department of Energy. The Office of Science is the single largest supporter of basic research in the physical sciences in the United States, and is working to address some of the most pressing challenges of our time. For more information, please visit science.energy.gov.

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How a Molecular Signal Helps Plant Cells Decide When to Make Oil

Scientists identify new details of how a sugar-signaling molecule helps regulate oil production in plant cells. The work could point to new ways to engineer plants to produce substantial amounts of oil for use as biofuels or in the production of other oil-based products.

Neutrons Produce First Direct 3D Maps of Water During Cell Membrane Fusion

New 3D maps of water distribution during cellular membrane fusion could lead to new treatments for diseases associated with cell fusion. Using neutron diffraction at Oak Ridge National Laboratory, scientists made the first direct observations of water in lipid bilayers modeling cell membrane fusion.

Chemists Demonstrate Sustainable Approach to Carbon Dioxide Capture From Air

Chemists at Oak Ridge National Laboratory have demonstrated a practical, energy-efficient method of capturing carbon dioxide directly from air. If deployed at large scale and coupled to geologic storage, the technique may bolster the portfolio of responses to global climate change.

Nucleation a boon to sustainable nanomanufacturing

Young-Shin Jun, professor of energy, environmental & chemical engineering in the School of Engineering & Applied Science, and Quingun Li, a former doctoral student in her lab, are the first to measure the activation energy and kinetic factors of calcium carbonate's nucleation, both key to predicting and controlling the process.

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Argonne scientists and their collaborators have developed a new model that merges basic electrochemical theory with theories used in different contexts, such as the study of photoelectrochemistry and semiconductor physics, to describe phenomena that occur in any electrode.

A prize-winning measurement device could aid a wide range of industries

Companies dealing with liquids ranging from wastewater to molten metals could benefit from a prize-winning device developed by researchers at the U.S. Department of Energy's (DOE) Princeton Plasma Physics Laboratory (PPPL) and Princeton University.

After 150 years, a Breakthrough in Understanding the Conversion of CO2 to Electrofuels

Using surface-enhanced Raman spectroscopy, Columbia Engineers are first to observe how CO2 is activated at the electrode-electrolyte interface; their finding shifts the catalyst design from trial-and-error paradigm to a rational approach and could lead to alternative, cheaper, and safer renewable energy storage.

Water Plays Unexpected Role in Forming Minerals

Water molecules line up tiny particles to attach and form minerals; understanding how this happens impacts energy extraction and storage along with waste disposal.

X-Rays Uncover a Hidden Property That Leads to Failure in a Lithium-Ion Battery Material

X-ray experiments at the Department of Energy's SLAC National Accelerator Laboratory and Lawrence Berkeley National Laboratory have revealed that the pathways lithium ions take through a common battery material are more complex than previously thought.


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Berkeley Lab to Build an Advanced Quantum Computing Testbed

Lawrence Berkeley National Laboratory (Berkeley Lab) will receive $30 million over five years from the U.S. Department of Energy to build and operate an Advanced Quantum Testbed (AQT) allowing researchers to explore superconducting quantum processors to advance scientific research

Cheng wins Midwest Energy News' 40 Under 40 Award

Lei Cheng, an assistant chemist in the Materials Science division at the U.S. Department of Energy's (DOE) Argonne National Laboratory, has received a Midwest Energy News 40 Under 40 Award.

JCESR renewed for another five years

The U.S. Department of Energy (DOE) today announced its decision to renew the Joint Center for Energy Storage Research (JCESR), a DOE Energy Innovation Hub led by Argonne National Laboratory and focused on advancing battery science and technology.

Binghamton designated as NextFlex New York Node for flexible hybrid electronics initiative

NextFlex has designated Binghamton University to be the New York "Node" for its flexible hybrid electronics (FHE) initiative. As the NextFlex New York Node, Binghamton will design, develop and manufacture tools; process materials and products for flexible hybrid electronics; and attract, train and employ an advanced manufacturing workforce, building on the region's existing electronics manufacturing base.

First Particle Tracks Seen in Prototype for International Neutrino Experiment

The largest liquid-argon neutrino detector in the world has just recorded its first particle tracks, signaling the start of a new chapter in the story of the international Deep Underground Neutrino Experiment (DUNE). DUNE's scientific mission is dedicated to unlocking the mysteries of neutrinos, the most abundant (and most mysterious) matter particles in the universe.

Tais Gorkhover Wins LCLS Young Investigator Award for Pioneering Novel X-ray Imaging Methods

Tais Gorkhover, a principal investigator with the Stanford PULSE Institute, will receive the 2018 LCLS Young Investigator Award, granted to early-career scientists in recognition of exceptional research using the Linac Coherent Light Source (LCLS) X-ray free-electron laser at the Department of Energy's SLAC National Accelerator Laboratory.

ORNL, United Kingdom Lab Partner on Nuclear Energy Research

The United Kingdom's National Nuclear Laboratory and the U.S. Department of Energy's Oak Ridge National Laboratory have agreed to cooperate on a wide range of nuclear energy research and development efforts that leverage both organizations' unique expertise and capabilities.

Nat Fisch receives Fusion Power Associates' Distinguished Career Award

Feature describes lifetime career award for PPPL physicist and professor Nat Fisch.

Wells Fargo Innovation Incubator Expands Focus to Include the Food-Water-Energy Interconnection

The Wells Fargo Innovation Incubator (IN2), a technology incubator and platform funded by the Wells Fargo Foundation and administered by the National Renewable Energy Laboratory (NREL), is expanding its program to advance technologies that address the interconnection of food, water and energy.

Graham George receives Lytle Award for contributions to X-ray absorption spectroscopy

Graham Neil George, professor and Canada Research Chair in X-ray Absorption Spectroscopy (XAS) at the University of Saskatchewan, has been chosen to receive the 2018 Farrel W. Lytle Award for his outstanding contributions to synchrotron science at the Department of Energy's SLAC National Accelerator Laboratory.


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Breaking the Symmetry Between Fundamental Forces

Scientists improve our understanding of the relationship between fundamental forces by re-creating the earliest moments of the universe.

Water Plays Unexpected Role in Forming Minerals

Water molecules line up tiny particles to attach and form minerals; understanding how this happens impacts energy extraction and storage along with waste disposal.

Heavy Particles Get Caught Up in the Flow

First direct measurement show how heavy particles containing a charm quark get caught up in the flow of early universe particle soup.

Seeing Between the Atoms

New detector enables electron microscope imaging at record-breaking resolution.

Scaling Up Single-Crystal Graphene

New method can make films of atomically thin carbon that are over a foot long.

Discovered: Optimal Magnetic Fields Suppress Instabilities in Tokamak Plasmas

U.S. and Korean scientists show how to find and use beneficial 3-D field perturbations to stabilize dangerous edge-localized modes in plasma.

New Electron Glasses Sharpen Our View of Atomic-Scale Features

A new approach to atom probe tomography promises more precise and accurate measurements vital to semiconductors used in computers, lasers, detectors, and more.

Getting an Up-Close, 3-D View of Gold Nanostars

Scientists can now measure 3-D structures of tiny particles with properties that hold promise for advanced sensors and diagnostics.

Small, Short-Lived Drops of Early Universe Matter

Particle flow patterns suggest even small-scale collisions create drops of early universe quark-gluon plasma.

Tuning Terahertz Beams with Nanoparticles

Scientists uncover a way to control terahertz radiation using tiny engineered particles in a magnetic field, potentially opening the doors for better medical and environmental sensors.


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