Credit: Proceedings of the National Academy of Sciences
Sequential intravital multiphoton microscopy of an autofluorescence chemotherapeutic agent (red) in BT474-Gluc brain tumors (green) after FUS-mediated blood-brain barrier disruption (FUS-BBBD). White arrows show increased drug extravasation and penetration after FUS-BBBD (white arrows). Yellow arrows show increased doxorubicin transmembrane transport in endothelial cells after FUS-BBBD. The bottom left and right images were collected 2 and 5 minutes after drug administration respectively. Scale bar 30 µm.