DOE News
    Doe Science news source
    The DOE Science News Source is a Newswise initiative to promote research news from the Office of Science of the DOE to the public and news media.
    • 2015-05-21 14:05:00
    • Article ID: 634683

    Researchers Watch Protein 'Quake' after Chemical Bond Break

    X-ray Laser Experiment Offers New Views into Ultrafast Motions in Life's Microscopic Machinery

    • Credit: SLAC National Accelerator Laboratory

      This computerized animation provides an artistic view of a quaking motion in myoglobin, a protein found in our muscles. The sphere toward the lower left of the protein represents an iron atom that serves as the “epicenter” for a light-triggered quaking motion observed in an experiment at SLAC’s Linac Coherent Light Source, an X-ray free-electron laser.

    • Credit: Nature Communications

      This computerized rendering shows the 3-D structure of myoglobin, an oxygen-storing protein found in many mammals’ muscles. The jagged green line represents a pulse of laser light that excites an iron atom (red sphere) at the core of the protein. The iron atom is at the “epicenter” (dotted blue line) of a quaking effect that propagates through the entire protein. Various parts of the protein involved in this motion are labeled A-H. The green sphere shows how far the iron atom moves during this shaking process.

    Scientists for the first time have precisely measured a protein’s natural “knee-jerk” reaction to the breaking of a chemical bond – a quaking motion that propagated through the protein at the speed of sound.

    The result, from an X-ray laser experiment at the Department of Energy’s SLAC National Accelerator Laboratory, could provide clues to how more complex processes unfold as chemical bonds form and break.

    “This work helps us to see how proteins work, in general,” said Marco Cammarata, who led the experiment at SLAC’s Linac Coherent Light Source (LCLS) X-ray laser, a DOE Office of Science User Facility. The research is detailed in the April 2 edition of Nature Communications.

    The study focused on myoglobin, a protein that stores oxygen in muscle cells. It is considered a model protein that is sometimes referred to as the “hydrogen atom of biology.” The unique LCLS pulses enabled researches to measure, for the first time at such an ultrafast timescale and under very natural conditions, how the entire protein shook in response to a light-triggered break in a molecular bond.

    Proteins are a vital part of the body’s microscopic machinery. Their movement and shape help determine their function, and studying them is key in designing new drugs to fight disease, for example, and in replicating natural systems to produce new fuel sources. Understanding the natural motion of proteins in response to such basic and essential biochemical reactions as bond breaks could provide insight about a range of biological processes.

    Watching a ‘Protein Quake’

    Scientists had previously seen some evidence of a quaking motion in a bacterial protein jarred by much higher energies, and had also observed myoglobin motion at longer timescales.

    In the LCLS experiment they triggered the quake by attaching carbon monoxide molecules to the myoglobin and then breaking this bond with a light pulse. They then hit the samples with LCLS X-rays, and varied the timing of the X-rays to view the triggered motion.

    Within a fraction of a picosecond, or trillionth of a second, after the bond broke, a quake-like wave rolled across the entire protein, causing it to stretch out.

    The rapid expansion, which researchers precisely measured from the scattering of ultrabright X-rays that hit the samples, was followed by a vigorous shaking, like rustling leaves on a jounced tree limb.

    They found that this quake-like effect, which had its “epicenter” at an iron atom at the core of the protein, dies off after just a few picoseconds. Based on their X-ray observations of the expansion and oscillations, researchers are working on a 3-D model detailing the protein’s motion.

    New Clues to Protein Function

    The way the protein responds to the shaking motion could provide clues to how myoglobin functions in the body.

    “This could suggest how the protein opens up channels to let oxygen in and out,” said Henrik Lemke, an LCLS staff scientist who participated in the experiment. A similar oxygen-release process occurs in photosynthesis.

    Cammarata said follow-up experiments, using a combination of X-ray techniques, will help determine whether more complex proteins, including other metal-containing proteins, exhibit the same kind of response as myoglobin.

    “We’ll be able to see if this type of quaking is a feature common to other proteins, too,” he said.

    Researchers participating in the experiment were from SLAC’s LCLS, University of Palermo in Italy, Institute of Structural Biology and University of Rennes in France, and Institute for Basic Science and KAIST in South Korea. The work was supported by Exploratory Projects Premier Support (PEPS) project “SASLELX” of the CNRS and University of Rennes in France, the University of Palermo in Italy, and the Institute for Basic Science in Korea.

    SLAC is a multi-program laboratory exploring frontier questions in photon science, astrophysics, particle physics and accelerator research. Located in Menlo Park, Calif., SLAC is operated by Stanford University for the U.S. Department of Energy’s Office of Science. For more information, please visit slac.stanford.edu.

    SLAC National Accelerator Laboratory is supported by the Office of Science of the U.S. Department of Energy. The Office of Science is the single largest supporter of basic research in the physical sciences in the United States, and is working to address some of the most pressing challenges of our time. For more information, please visit science.energy.gov.

    • other-fb
    • other-tw
    X
    X
    X
    • Filters

    • × Clear Filters
    Sea Quark Spin Surprise!

    Sea Quark Spin Surprise!

    Antiquark spin contribution to proton spin depends on flavor, which could help unlock secrets about the nuclear structure of atoms that make up nearly all visible matter in our universe.

    The Weak Side of the Proton

    The Weak Side of the Proton

    A precision measurement of the proton's weak charge narrows the search for new physics.

    CEBAF Turns on the Charm

    CEBAF Turns on the Charm

    The world's most advanced particle accelerator for investigating the quark structure of the atom's nucleus has just charmed physicists with a new capability. The production of charm quarks in J/ψ (J/psi) particles by CEBAF at the Department of Energy's Thomas Jefferson National Accelerator Facility confirms that the facility has expanded the realm of precision nuclear physics research with electron beams to higher energies.

    Fast-Moving Pairs May Solve 35-Year-Old Mystery

    Fast-Moving Pairs May Solve 35-Year-Old Mystery

    Physicists develop a universal mathematical description that suggests that proton-neutron pairs in a nucleus may explain why their associated quarks have lower average momenta than predicted.

    Artificial intelligence accelerates efforts to develop clean, virtually limitless fusion energy

    Artificial intelligence accelerates efforts to develop clean, virtually limitless fusion energy

    Feature describes Nature paper on opening a new chapter in fusion research with artificial intelligence.

    Team Takes Fluoride from Taps and Toothpaste to Batteries

    Team Takes Fluoride from Taps and Toothpaste to Batteries

    With user facilities, researchers devise novel battery chemistries to help make fluoride batteries a reality.

    Quarks Under Pressure in the Proton

    Quarks Under Pressure in the Proton

    Pressure in the middle of a proton is about 10 times higher than in a neutron star.

    Physicists Improve Understanding of Heat and Particle Flow in the Edge of a Fusion Device

    Physicists Improve Understanding of Heat and Particle Flow in the Edge of a Fusion Device

    PPPL physicists have discovered valuable information about how plasma flows at the edge inside doughnut-shaped fusion devices. The findings mark an encouraging sign for the development of machines to produce fusion energy for generating electricity without creating long-term hazardous waste.

    Magnetic Levitation of Ultracold Neutrons Yields New Measurement of the Neutron Lifetime

    Magnetic Levitation of Ultracold Neutrons Yields New Measurement of the Neutron Lifetime

    Storing extremely slow neutrons in a novel trap enables precise measurement of a basic property of particle physics.

    SLAC's High-Speed 'Electron Camera' Films Molecular Movie in HD

    SLAC's High-Speed 'Electron Camera' Films Molecular Movie in HD

    With an extremely fast "electron camera" at the Department of Energy's SLAC National Accelerator Laboratory, researchers have made the first high-definition "movie" of ring-shaped molecules breaking open in response to light. The results could further our understanding of similar reactions with vital roles in chemistry, such as the production of vitamin D in our bodies.


    • Filters

    • × Clear Filters

    Department of Energy Announces $20 Million for Artificial Intelligence Research

    Today, the U.S. Department of Energy (DOE) announced a total of $20 million in funding for innovative research and development in artificial intelligence (A.I.) and machine learning.

    Tim Knewitz named Argonne National Laboratory's Chief Financial Officer

    Tim Knewitz named Argonne National Laboratory's Chief Financial Officer

    The U.S. Department of Energy's Argonne National Laboratory has named Tim Knewitz at its Chief Financial Officer.

    Department of Energy Announces $95 Million for Small Business Research and Development Grants

    U.S. Energy Secretary Rick Perry today announced that the Department of Energy will award 86 grants totaling $95 million to 74 small businesses in 21 states.

    DOE's Science Graduate Student Research Program Selects 70 Students to Pursue Research at DOE Laboratories

    The Department of Energy's (DOE's) Office of Science has selected 70 graduate students from across the nation for its 2018 Solicitation 2 cycle for Office of Science Graduate Student Research (SCGSR) Program.

    Brookhaven Joins the IBM Q Network Hub at Oak Ridge National Lab

    Brookhaven Joins the IBM Q Network Hub at Oak Ridge National Lab

    Brookhaven National Lab has joined the IBM Q Network Hub at Oak Ridge National Lab. This hub is part of an international community of Fortune 500 companies, startups, universities, and research labs working with IBM to advance quantum computing and explore its practical applications.

    David Reis named head of PULSE Institute for ultrafast science

    David Reis named head of PULSE Institute for ultrafast science

    Long before David Reis joined the faculty of the Department of Energy's SLAC National Accelerator Laboratory and Stanford University, he was helping lay the groundwork for the lab's first-of-a-kind X-ray free-electron laser, or XFEL, and the revolutionary science that followed its opening in 2009. Now he's director of the PULSE Institute, which was founded by SLAC and Stanford with the express purpose of exploiting the possibilities for ultrafast science at that X-ray laser, the Linac Coherent Light Source (LCLS).

    Head of NSTX-U research is appointed deputy director for research at the Princeton Plasma Physics Laboratory

    Head of NSTX-U research is appointed deputy director for research at the Princeton Plasma Physics Laboratory

    Jon Menard, the head of research on the Princeton Plasma Physics Laboratory's National Spherical Torus Experiment-Upgrade, has been named deputy director for research. Michael Zarnstorff, who held the position for 10 years, will become the chief chief scientist at PPPL, a position that will oversee strategic scientific planning.

    Argonne scientist advances energy sciences through professional leadership

    Argonne scientist advances energy sciences through professional leadership

    Ralph Muehleisen of the U.S. Department of Energy's (DOE) Argonne National Laboratory was recently re-elected to the Board of Directors of IBPSA-USA, the U.S. affiliate of the International Building Performance Simulation Association. IBPSA is a global leader in the promotion of building simulation science and one of the largest professional organizations in the world for building scientists and engineers.

    Brookhaven Lab Publishes Second Edition of Nuclear Nonproliferation Textbook

    Brookhaven Lab Publishes Second Edition of Nuclear Nonproliferation Textbook

    Brookhaven Lab has published the second edition of Deterring Nuclear Proliferation: The Importance of IAEA Safeguards, a textbook that provides a history of the origins of the International Atomic Energy Agency (IAEA) and introduces the ways in which IAEA verifies nation states' nuclear nonproliferation commitments.

    PPPL's Young Women's Conference Offers Girls Fun and Inspiration in STEM Fields

    PPPL's Young Women's Conference Offers Girls Fun and Inspiration in STEM Fields

    PPPL's Young Women's Conference in Science, Technology, Engineering and Mathematics on Friday, March 22, at Princeton University, seeks to change the statistics that show women still lag far behind men in the STEM fields. The conference offers 7th to 10th-grade girls hands-on science activities, exciting experiments, and talks and a keynote speech by early-career female scientists.


    • Filters

    • × Clear Filters
    Sea Quark Spin Surprise!

    Sea Quark Spin Surprise!

    Antiquark spin contribution to proton spin depends on flavor, which could help unlock secrets about the nuclear structure of atoms that make up nearly all visible matter in our universe.

    The Weak Side of the Proton

    The Weak Side of the Proton

    A precision measurement of the proton's weak charge narrows the search for new physics.

    Fast-Moving Pairs May Solve 35-Year-Old Mystery

    Fast-Moving Pairs May Solve 35-Year-Old Mystery

    Physicists develop a universal mathematical description that suggests that proton-neutron pairs in a nucleus may explain why their associated quarks have lower average momenta than predicted.

    Team Takes Fluoride from Taps and Toothpaste to Batteries

    Team Takes Fluoride from Taps and Toothpaste to Batteries

    With user facilities, researchers devise novel battery chemistries to help make fluoride batteries a reality.

    Quarks Under Pressure in the Proton

    Quarks Under Pressure in the Proton

    Pressure in the middle of a proton is about 10 times higher than in a neutron star.

    Magnetic Levitation of Ultracold Neutrons Yields New Measurement of the Neutron Lifetime

    Magnetic Levitation of Ultracold Neutrons Yields New Measurement of the Neutron Lifetime

    Storing extremely slow neutrons in a novel trap enables precise measurement of a basic property of particle physics.

    New Molecular Blueprint Aids Study of Photosynthesis

    New Molecular Blueprint Aids Study of Photosynthesis

    Insights into how nature converts carbon dioxide into sugar could help scientists develop crops that produce fuels and other products.

    Catching Fast Changes in Excited Molecules

    Catching Fast Changes in Excited Molecules

    Scientists observe and control molecular and atomic dynamics at the fastest timescales to date.

    Atomic Maps Reveal How Iron Rusts

    Atomic Maps Reveal How Iron Rusts

    Scientists discovered how iron atoms continually re-arrange on surfaces, offering insights into metal corrosion and soil remediation.

    Strain and Defects Grow in Tiny Magnetite Crystals When Oxidized

    Strain and Defects Grow in Tiny Magnetite Crystals When Oxidized

    Detailed 3D images show how nanoparticles change in reactions that purify contaminated water or power recyclable geochemical batteries.


    Spotlight

    DOE's Science Graduate Student Research Program Selects 70 Students to Pursue Research at DOE Laboratories
    Friday April 12, 2019, 03:05 PM

    DOE's Science Graduate Student Research Program Selects 70 Students to Pursue Research at DOE Laboratories

    Department of Energy, Office of Science

    Young Women's Conference in STEM seeks to change the statistics one girl at a time
    Thursday March 28, 2019, 03:05 PM

    Young Women's Conference in STEM seeks to change the statistics one girl at a time

    Princeton Plasma Physics Laboratory

    Students team with Argonne scientists and engineers to learn about STEM careers
    Tuesday March 12, 2019, 05:05 PM

    Students team with Argonne scientists and engineers to learn about STEM careers

    Argonne National Laboratory

    Lynbrook High wins 2019 SLAC Regional Science Bowl competition
    Wednesday February 13, 2019, 02:05 PM

    Lynbrook High wins 2019 SLAC Regional Science Bowl competition

    SLAC National Accelerator Laboratory

    Equipping the next generation for a technological revolution
    Thursday January 24, 2019, 01:05 PM

    Equipping the next generation for a technological revolution

    Argonne National Laboratory

    Chemistry intern inspired by Argonne's real-world science
    Friday January 18, 2019, 05:05 PM

    Chemistry intern inspired by Argonne's real-world science

    Argonne National Laboratory

    Chasing a supernova
    Friday January 18, 2019, 04:05 PM

    Chasing a supernova

    Argonne National Laboratory

    Argonne intern streamlines the beamline
    Tuesday January 08, 2019, 02:05 PM

    Argonne intern streamlines the beamline

    Argonne National Laboratory

    Research on Light-Matter Interaction Could Lead to Improved Electronic and Optoelectronic Devices
    Thursday October 11, 2018, 04:00 PM

    Research on Light-Matter Interaction Could Lead to Improved Electronic and Optoelectronic Devices

    Rensselaer Polytechnic Institute (RPI)

    Innovating Our Energy Future
    Wednesday October 03, 2018, 07:05 PM

    Innovating Our Energy Future

    Oregon State University, College of Engineering

    Physics graduate student takes her thesis research to a Department of Energy national lab
    Tuesday October 02, 2018, 03:05 PM

    Physics graduate student takes her thesis research to a Department of Energy national lab

    University of Alabama at Birmingham

    Friday September 21, 2018, 01:05 PM

    "Model" students enjoy Argonne campus life

    Argonne National Laboratory

    Writing Code for a More Skilled and Diverse STEM Workforce
    Thursday September 06, 2018, 01:05 PM

    Writing Code for a More Skilled and Diverse STEM Workforce

    Brookhaven National Laboratory

    New graduate student summer school launches at Princeton Plasma Physics Laboratory
    Tuesday September 04, 2018, 11:30 AM

    New graduate student summer school launches at Princeton Plasma Physics Laboratory

    Princeton Plasma Physics Laboratory

    The Gridlock State
    Friday August 31, 2018, 06:05 PM

    The Gridlock State

    California State University (CSU) Chancellor's Office

    Meet Jasmine Hatcher and Trishelle Copeland-Johnson
    Friday August 31, 2018, 02:05 PM

    Meet Jasmine Hatcher and Trishelle Copeland-Johnson

    Brookhaven National Laboratory

    Argonne hosts Modeling, Experimentation and Validation Summer School
    Friday August 24, 2018, 11:05 AM

    Argonne hosts Modeling, Experimentation and Validation Summer School

    Argonne National Laboratory

    Students affected by Hurricane Maria bring their research to SLAC
    Wednesday August 22, 2018, 01:05 PM

    Students affected by Hurricane Maria bring their research to SLAC

    SLAC National Accelerator Laboratory

    Brookhaven Lab Pays Tribute to 2018 Summer Interns
    Wednesday August 22, 2018, 10:05 AM

    Brookhaven Lab Pays Tribute to 2018 Summer Interns

    Brookhaven National Laboratory

    Changing How Buildings Are Made
    Monday August 20, 2018, 12:05 PM

    Changing How Buildings Are Made

    Washington University in St. Louis

    CSUMB Selected to Host Architecture at Zero Competition in 2019
    Thursday August 16, 2018, 12:05 PM

    CSUMB Selected to Host Architecture at Zero Competition in 2019

    California State University, Monterey Bay

    Department of Energy Invests $64 Million in Advanced Nuclear Technology
    Friday July 20, 2018, 03:00 PM

    Department of Energy Invests $64 Million in Advanced Nuclear Technology

    Rensselaer Polytechnic Institute (RPI)

    Professor Miao Yu Named the Priti and Mukesh Chatter '82 Career Development Professor
    Thursday July 19, 2018, 05:00 PM

    Professor Miao Yu Named the Priti and Mukesh Chatter '82 Career Development Professor

    Rensselaer Polytechnic Institute (RPI)

    2018 RHIC & AGS Annual Users' Meeting: 'Illuminating the QCD Landscape'
    Tuesday July 03, 2018, 11:05 AM

    2018 RHIC & AGS Annual Users' Meeting: 'Illuminating the QCD Landscape'

    Brookhaven National Laboratory

    Argonne welcomes <em>The Martian</em> author Andy Weir
    Friday June 29, 2018, 06:05 PM

    Argonne welcomes The Martian author Andy Weir

    Argonne National Laboratory

    Creating STEM Knowledge and Innovations to Solve Global Issues Like Water, Food, and Energy
    Monday June 18, 2018, 09:55 AM

    Creating STEM Knowledge and Innovations to Solve Global Issues Like Water, Food, and Energy

    Illinois Mathematics and Science Academy (IMSA)

    Professor Emily Liu Receives $1.8 Million DoE Award for Solar Power Systems Research
    Friday June 15, 2018, 10:00 AM

    Professor Emily Liu Receives $1.8 Million DoE Award for Solar Power Systems Research

    Rensselaer Polytechnic Institute (RPI)

    Celebrating 40 years of empowerment in science
    Thursday June 07, 2018, 03:05 PM

    Celebrating 40 years of empowerment in science

    Argonne National Laboratory

    Introducing Graduate Students Across the Globe to Photon Science
    Monday May 07, 2018, 10:30 AM

    Introducing Graduate Students Across the Globe to Photon Science

    Brookhaven National Laboratory

    Students from Massachusetts and Washington Win DOE's 28th National Science Bowl(r)
    Wednesday May 02, 2018, 04:05 PM

    Students from Massachusetts and Washington Win DOE's 28th National Science Bowl(r)

    Department of Energy, Office of Science

    The Race for Young Scientific Minds
    Thursday April 12, 2018, 07:05 PM

    The Race for Young Scientific Minds

    Argonne National Laboratory

    Q&A: Al Ashley Reflects on His Efforts to Diversify SLAC and Beyond
    Wednesday March 14, 2018, 02:05 PM

    Q&A: Al Ashley Reflects on His Efforts to Diversify SLAC and Beyond

    SLAC National Accelerator Laboratory

    Insights on Innovation in Energy, Humanitarian Aid Highlight UVA Darden's Net Impact Week
    Thursday February 15, 2018, 12:05 PM

    Insights on Innovation in Energy, Humanitarian Aid Highlight UVA Darden's Net Impact Week

    University of Virginia Darden School of Business

    Ivy League Graduate, Writer and Activist with Dyslexia Visits CSUCI to Reframe the Concept of Learning Disabilities
    Friday February 09, 2018, 11:05 AM

    Ivy League Graduate, Writer and Activist with Dyslexia Visits CSUCI to Reframe the Concept of Learning Disabilities

    California State University, Channel Islands

    Photographer Adam Nadel Selected as Fermilab's New Artist-in-Residence for 2018
    Wednesday January 17, 2018, 12:05 PM

    Photographer Adam Nadel Selected as Fermilab's New Artist-in-Residence for 2018

    Fermi National Accelerator Laboratory (Fermilab)

    Fermilab Computing Partners with Argonne, Local Schools for Hour of Code
    Wednesday January 17, 2018, 12:05 PM

    Fermilab Computing Partners with Argonne, Local Schools for Hour of Code

    Fermi National Accelerator Laboratory (Fermilab)

    Q&A: Sam Webb Teaches X-Ray Science from a Remote Classroom
    Wednesday December 20, 2017, 01:05 PM

    Q&A: Sam Webb Teaches X-Ray Science from a Remote Classroom

    SLAC National Accelerator Laboratory

    The Future of Today's Electric Power Systems
    Monday December 18, 2017, 01:05 PM

    The Future of Today's Electric Power Systems

    Rensselaer Polytechnic Institute (RPI)

    Supporting the Development of Offshore Wind Power Plants
    Monday December 18, 2017, 12:05 PM

    Supporting the Development of Offshore Wind Power Plants

    Rensselaer Polytechnic Institute (RPI)

    Stairway to Science
    Tuesday October 03, 2017, 01:05 PM

    Stairway to Science

    Argonne National Laboratory

    After-School Energy Rush
    Thursday September 28, 2017, 12:05 PM

    After-School Energy Rush

    Argonne National Laboratory

    Bringing Diversity Into Computational Science Through Student Outreach
    Thursday September 28, 2017, 10:05 AM

    Bringing Diversity Into Computational Science Through Student Outreach

    Brookhaven National Laboratory

    From Science to Finance: SLAC Summer Interns Forge New Paths in STEM
    Thursday September 21, 2017, 03:05 PM

    From Science to Finance: SLAC Summer Interns Forge New Paths in STEM

    SLAC National Accelerator Laboratory

    Students Discuss 'Cosmic Opportunities' at 45th Annual SLAC Summer Institute
    Thursday September 07, 2017, 02:05 PM

    Students Discuss 'Cosmic Opportunities' at 45th Annual SLAC Summer Institute

    SLAC National Accelerator Laboratory

    Binghamton University Opens $70 Million Smart Energy Building
    Thursday August 31, 2017, 05:05 PM

    Binghamton University Opens $70 Million Smart Energy Building

    Binghamton University, State University of New York

    Widening Horizons for High Schoolers with Code
    Wednesday August 23, 2017, 05:05 PM

    Widening Horizons for High Schoolers with Code

    Argonne National Laboratory





    Showing results

    0-4 Of 2215