Machine learning sheds light on gene transcription

DALLAS – May 13, 2024 – A team led by researchers at UT Southwestern Medical Center developed deep learning models to identify a simple set of rules that govern the activity of promoters – regions of DNA that initiate the process by which genes produce proteins. Their findings, published in Science, could lead to a better understanding of how promoters contribute to gene regulation in health and disease.

“Although promoters are essential for the function of every gene, our understanding of how these genetic elements operate is incomplete despite decades of study that have defined many of their features. Our research sheds new light on how these sequences work in humans and other mammals,” said Jian Zhou, Ph.D., Assistant Professor in the Lyda Hill Department of Bioinformatics at UT Southwestern. Dr. Zhou co-led the study with first author Kseniia Dudnyk, a graduate student in the Zhou Lab, and Jian Xu, Ph.D., a former researcher at the Children’s Medical Center Research Institute at UT Southwestern.

Creating the proteins that cells use to perform their activities starts with a process known as transcription. That’s when an RNA polymerase protein latches onto a DNA strand and copies – or transcribes – the encoded information into an RNA molecule. The region where the RNA polymerase attaches to begin transcription is called the promoter. In humans, promoters are typically composed of hundreds of base pairs, the units that make up DNA. Although researchers have identified common base pair sequences shared among some regions of DNA that are promoters, these sequences are often absent in human promoters, leaving the rules of how DNA sequence directs the transcription process unclear.

To better define promoters in humans and how they operate, the researchers developed a machine learning program they named Puffin. After analyzing data from tens of thousands of recognized human promoters, the program determined that they are made of three types of sequence patterns: motifs, initiators, and trinucleotides.

Puffin showed that depending on how these elements are arranged, they can activate or repress transcription of a gene. Puffin also can predict how the arrangement of these elements can direct RNA polymerase to preferentially transcribe a single strand of DNA or transcribe both strands simultaneously toward opposite directions. This bidirectional transcription is common in human genes.

The program further showed that mice and other mammals shared similar rule sets for governing promoter operation. In addition, Puffin allowed the researchers to predict whether and how transcription would occur if they mutated promoters, findings that closely matched those from experiments.

The study authors suggested that Puffin could help them understand how promoters work in healthy cells as well as how disease-associated alterations in promoters could lead to changes in gene transcription. This program is available on a free web server (tss.zhoulab.io) so that other researchers can test any promoter sequence of interest. They added that using a similar machine learning approach could offer insights into other facets of the genome that are still not well understood.

Additional UTSW researchers who contributed to this study are Donghong Cai, Ph.D., a joint postdoctoral fellow with the Xu Lab, and Chenlai Shi, M.S., Data Scientist.

This study was funded by grants from the National Institutes of Health (DP2GM146336, R01DK111430, R01CA230631, and R01CA259581); the Cancer Prevention and Research Institute of Texas (RR190071); and a Leukemia & Lymphoma Society Scholar award.

Dr. Zhou is a Lupe Murchison Foundation Scholar in Medical Research and a member of the Harold C. Simmons Comprehensive Cancer Center.

About UT Southwestern Medical Center  

UT Southwestern, one of the nation’s premier academic medical centers, integrates pioneering biomedical research with exceptional clinical care and education. The institution’s faculty members have received six Nobel Prizes and include 25 members of the National Academy of Sciences, 21 members of the National Academy of Medicine, and 13 Howard Hughes Medical Institute Investigators. The full-time faculty of more than 3,100 is responsible for groundbreaking medical advances and is committed to translating science-driven research quickly to new clinical treatments. UT Southwestern physicians provide care in more than 80 specialties to more than 120,000 hospitalized patients, more than 360,000 emergency room cases, and oversee nearly 5 million outpatient visits a year.