Newswise — BOSTON – (March 25, 2021) – A mechanism has been identified that explains how physical exercise in pregnancy confers metabolic health benefits in offspring. According to researchers, the key lies with a protein called SOD3, vitamin D and adequate exercise, with the outcomes possibly forming the first steps to designing rational diet and exercise programs to use during pregnancy and particularly when mothers may also be overweight or obese.

The study, which was led by authors from the Joslin Diabetes Center at the Harvard Medical School and colleagues from Japan, the US, Canada and Denmark, has been published online by Cell Metabolism .

“We’ve known for a while that risks for obesity and type 2 diabetes can originate in the critical prenatal developmental period,” said senior author Laurie Goodyear. “In particular, there is real concern that the increasing levels of obesity seen in women of reproductive age will transmit disease risk to subsequent generations. It’s important to understand that if this is not alleviated, rates of diabetes and obesity will only continue to grow in the coming years.”

Many previous studies have linked increased maternal body weight and unhealthy diets to poorer metabolic outcomes in offspring, often many years later. Understanding the mechanisms of how maternal exercise can reverse these effects might lead to interventions that prevent these diseases transmitting across generations, say the authors of the study.

“The findings offer an explanation as to why physical exercise during pregnancy may have metabolic benefits for offspring as they get older,” said Goodyear. “We show how physical exercise during pregnancy, in combination with adequate vitamin D levels, enhances levels of a placenta-derived protein called SOD3 (superoxide dismutase 3), and that via a number of intermediate steps, this improves glucose tolerance in offspring.”

The findings come from a series of investigations with pregnant mice, comparing groups exposed to voluntary wheel running (i.e., exercise) and groups that were sedentary. Using various techniques, the authors carefully investigated the effects of exercise on parameters such as DNA methylation, cell signaling and gene expression, particularly in relation to glucose metabolism.

In short, they found that SOD3 is an exercise-induced placenta-derived protein that activates a specific signaling pathway that controls DNA demethylation in the livers of offspring, which in turn improves a number of aspects of glucose metabolism in offspring.

They also highlight the critical role of vitamin D as a mediator of SOD3 expression, with their data suggesting that maternal diet had to have sufficient vitamin D levels to influence placental levels of SOD3 and hence any improvements in metabolic health in offspring. They note for example, that high dietary levels of the vitamin in the absence of exercise, and indeed the opposite scenario, did not lead to increases in SOD3 - a consequential point if SOD3 is targeted clinically, they suggest.

Although most of the investigation focuses on mice, the authors did also look at SOD3 levels in pregnant women, finding that those who exercised more did have higher levels of serum and placental SOD3 and that they appeared to be highest during the second trimester of pregnancy.

“In terms of clinical application, it looks like the most efficient way to raise levels of the SOD3 protein is still likely to be via physical exercise,” said lead author Joji Kusuyama. He added that there may also be clinical interest in using maternal serum levels of SOD3 as a biomarker to assess the benefits of exercise during pregnancy.

“Our data hints that exercise in combination with optimal vitamin D levels might be particularly beneficial during the 2nd trimester of pregnancy,” said Kusuyama. “Although we can’t be definitive about this with the current findings, we now plan to look in detail at how diet and exercise type and timing might be optimized with maternal serum SOD3 measurements to obtain maximum benefits for offspring.”

The authors note some limitations with the study, including generalizability to all races and ethnicities and that there are still certain aspects of the signaling pathway that require further investigation. Nonetheless, they suggest their findings offer important insights into the role of exercise during pregnancy and the likely dynamic metabolic benefits for offspring.

“This report focuses on SOD3 and the metabolic benefits for offspring of maternal exercise, but there may be wider benefits of this protein on other body organs. For example, we are currently investigating the effects of maternal exercise and SOD3 on brain function in the offspring,” said Goodyear. “We are also doing more in-depth investigation of maternal exercise effects on placenta because we found there are a plethora of changes in this tissue, and these placental adaptations may also have life-long effects in offspring. It’s early days but understanding how exercise and fitness before and during pregnancy work may be the key to better health for subsequent generations.”

Other contributors to the research include Ana Barbara Alves-Wagner, Royce Conlin, Nathan Makarewicz, Brent Albertson, Noah Prince, Shio Kobayashi, Chisayo Kozuka, Magnus Møller, Mette Bjerre, Jens Fuglsang, Emily Miele, Roeland Middelbeek, Yang Xiudong, Yang Xia, Léa Garneau, Jayonta Bhattacharjee, Céline Aguer, Mary Elizabeth Patti, Michael Hirshman, Niels Jessen, Toshihisa Hatta, Per Glud Ovesen, Kristi Adamo, and Eva Nozik-Grayck.

Funding for the study was provided by US National Institutes of Health, the American Diabetes Association, the Canadian Institutes of Health Research as well as many other organizations. Full details are available in the Cell Metabolism report.

About Joslin Diabetes Center

Joslin Diabetes Center is world-renowned for its deep expertise in diabetes treatment and research. Joslin is dedicated to finding a cure for diabetes and ensuring that people with diabetes live long, healthy lives. We develop and disseminate innovative patient therapies and scientific discoveries throughout the world. Joslin is an independent, non-profit institution affiliated with Harvard Medical School, and one of only 16 NIH-designated Diabetes Research Centers in the U.S.

For more information about the Joslin Diabetes Center, visit or follow @joslindiabetes | One Joslin Place, Boston, MA 617-309-2400

For more information about the study, the corresponding authors are Dr Laurie J. Goodyear ( [at] ()) and Dr Joji Kusuyama (joji.kusuyama.c1 [at] ()).

Kusuyama et al. Placental superoxide dismutase 3 mediates benefits of maternal exercise on offspring health. Cell Metabolism 2021. DOI: 10.1016/j.cmet.2021.03.004


Journal Link: Cell Metabolism