Wayne State University Division of Research

Wayne State University team receives $1.98 million NIH award to develop diagnostic tests for sarcoidosis

Newswise — DETROIT – Sarcoidosis is an inflammatory disease of unknown causes that affects multiple organs in the body. It occurs in patients around the world and is highly prevalent in Detroit and Michigan. It is characterized by abnormal masses or nodules – granuloma formations – in various organs, including lungs and lymph glands, brains and heart. Sarcoidosis has been described for more than 150 years, but there are no specific and simple tests developed to diagnose this disease.

Currently, sarcoidosis is diagnosed through confirmation of non-caseating granulomas in tissue biopsies of organs where there is no other applicable diagnosis. Sarcoidosis is similar to tuberculosis, which is an infectious granulomatous disease. Active tuberculosis infection can be deadly. Exposure to bacterial tuberculosis may stay dormant for long time and is called latent tuberculosis. One third of humans worldwide are infected with latent tuberculosis but have no symptoms. Under specific circumstances, such as immune suppression, these individuals may develop full-blown tuberculosis. There are no simple tests to discover latent tuberculosis infection from tuberculosis disease or from sarcoidosis. 

A team of researchers led by Lobelia Samavati, M.D., associate professor in the Center for Molecular Medicine and Genetics and Department of Internal Medicine at Wayne State University’s School of Medicine, has been working for more than 10 years to discover specific serological biomarkers of sarcoidosis and tuberculosis.

With the help of a recent $1.98-million grant from the National Heart, Lung, and Blood Institute of the National Institutes of Health, Samavati’s research team aims to advance their work of developing biomarker technology for identification of biomarkers of sarcoidosis.  

Samavati developed a comprehensive library derived from sarcoidosis tissue that can differentiate between sarcoidosis and several other respiratory disorders. Using a high throughput method, her team developed a complex epitope library derived from materials of sarcoidosis patients. This cDNA library contains large numbers of epitopes immunoscreened with sera from patients with sarcoidosis containing high titer IgG antibodies and the cloned phages, which have been used to construct an antigen microarray to detect antibodies against sarcoid antigen(s) in the sera of test subjects.

“We have identified a panel of biomarkers/classifiers with high sensitivity and specificity that can discriminate between sera of patients with sarcoidosis and healthy controls, as well as active tuberculosis subjects,” said Samavati. “Our results have been published in several peer-reviewed journals, and we have two different licenses for our invention.”

Samavati’s grant, “A Novel T7 Phage Display Technology to Detect Sarcoidosis Specific Antigens,” aims to advance and refine her technology to discriminate sarcoidosis from latent tuberculosis and healthy controls. Her team has identified novel antibodies that may be used as a molecular therapy target, and their goal over the next four years is to further map these peptides and identify their biological function. Their current data indicates that some of these peptides can be used as diagnostic tools, while others can be used as therapy targets.

The overall goal is to define the specific antigens initiating granuloma formation in sarcoidosis and how immunity in response to antigen leads to sarcoidosis and latent tuberculosis. In the future, this approach could be used to identify a panel of biomarkers useful for diagnosis of various organ involvements in sarcoidosis and differential diagnosis of other granulomatous diseases or response to treatment in sarcoidosis subjects. Furthermore, the identified antigens can be used to develop a model for sarcoidosis.

Using a high-throughput method, Samavati and her research team developed a novel platform based on T7 phage library — a cDNA library derived from mRNA isolated from bronchoalveolar lavage (BAL) and leucocytes of sarcoidosis patients. They constructed a microarray platform and immunoscreened with sarcoidosis subjects, tuberculosis, healthy controls and other respiratory diseases.

The phage display technology and immunoscreening have utilities not only in identification of diagnostic biomarkers, but also may enable Samavati and others to develop a novel targeted therapy utilizing the peptide sequences (mimotopes) as a vehicle to deliver specific drugs.

“We believe that our technology will be able to harness the diversity of antibodies and can aid to identify protective antibodies in various diseases in humans, including viral respiratory infections such as the corona virus,” said Samavati. “We believe that this study is the beginning of new era to identify protective immunity in form of antibodies.

Sorin Draghici, the Robert J. Sokol, M.D. Endowed Chair in Systems Biology in Reproduction and professor of computer science in Wayne State’s College of Engineering, is collaborating with Samavati. He contributed to the design of the study and will supervise the data analysis.

The grant number for this National Institutes of Health project is HL150474.

# # #

Wayne State University is one of the nation’s pre-eminent public research institutions in an urban setting. Through its multidisciplinary approach to research and education, and its ongoing collaboration with government, industry and other institutions, the university seeks to enhance economic growth and improve the quality of life in the city of Detroit, the state of Michigan and throughout the world. For more information about research at Wayne State University, visit http://www.research.wayne.edu.

Filters close

Showing results

110 of 2835
Released: 7-Aug-2020 2:25 PM EDT
Alcoholism treatment is potentially effective against COVID-19
National Research University - Higher School of Economics (HSE)

A team of chemists from HSE University and the Zelinsky Institute of Organic Chemistry used molecular modelling to find out that two medications that have been known for a long time can be used to fight SARS-CoV-2.

Newswise: 239651_web.jpg
Released: 7-Aug-2020 1:10 PM EDT
Electric cooker an easy, efficient way to sanitize N95 masks, study finds
University of Illinois at Urbana-Champaign

Owners of electric multicookers may be able to add another use to its list of functions, a new study suggests: sanitization of N95 respirator masks.

Released: 7-Aug-2020 12:05 PM EDT
Study: Most Americans don't have enough assets to withstand 3 months without income
Oregon State University

A new study from Oregon State University found that 77% of low- to moderate-income American households fall below the asset poverty threshold, meaning that if their income were cut off they would not have the financial assets to maintain at least poverty-level status for three months.

Released: 7-Aug-2020 11:55 AM EDT
COVID recovery choices shape future climate
University of Leeds

A post-lockdown economic recovery plan that incorporates and emphasises climate-friendly choices could help significantly in the battle against global warming, according to a new study.

access_time Embargo lifts in 2 days
Embargo will expire: 11-Aug-2020 11:00 AM EDT Released to reporters: 7-Aug-2020 10:55 AM EDT

A reporter's PressPass is required to access this story until the embargo expires on 11-Aug-2020 11:00 AM EDT The Newswise PressPass gives verified journalists access to embargoed stories. Please log in to complete a presspass application. If you have not yet registered, please Register. When you fill out the registration form, please identify yourself as a reporter in order to advance to the presspass application form.

Released: 7-Aug-2020 9:45 AM EDT
Potentially predictive humoral immune response markers in COVID-19 patients
Massachusetts General Hospital

Galit Alter, PhD, Group Leader at the Ragon Institute of MGH, MIT and Harvard and Professor of Medicine at Harvard Medical School, and Helen Chu, MD, Associate Professor of Medicine, Division of Allergy and Infectious Diseases, University of Washington School of Medicine, and UW Medicine physician, have recently published a paper which identifies five immune response markers which, collectively, were able to correctly classify both convalescent COVID-19 patients and those who did not survive the disease

Released: 7-Aug-2020 9:00 AM EDT
ACSM Publishes Call to Action Addressing COVID-19 and Return to Sports and Physical Activity
American College of Sports Medicine (ACSM)

ACSM published a call to action statement addressing COVID-19 and safely returning to sports and exercise. Authored by ACSM subject matter experts, the statement highlights the current science around COVID-19 and provides 12 action steps to consider. “COVID-19: Considerations for Sports and Physical Activity” is ACSM’s first call to action statement and published in the August issue of Current Sports Medicine Reports.

Newswise: University of Miami Sports Medicine Institute expert leads call to action for harnessing exercise’s health benefits during the pandemic
7-Aug-2020 9:00 AM EDT
University of Miami Sports Medicine Institute expert leads call to action for harnessing exercise’s health benefits during the pandemic
University of Miami Health System, Miller School of Medicine

To address and overcome the challenges so Americans can return to or sustain physical activity safely, Thomas M. Best, M.D., Ph.D., FACSM, professor of orthopedic surgery at the University of Miami Miller School of Medicine and research director of the UHealth Sports Medicine Institute, and sports medicine colleagues from around the U.S. wrote “COVID-19: Considerations for Sports and Physical Activity,” published August 7 in Current Sports Medicine Reports, an American College of Sports Medicine journal.

Released: 6-Aug-2020 3:10 PM EDT
AANA Supports Improvements to Rural Health Access
American Association of Nurse Anesthetists (AANA)

To provide high-quality, value-based healthcare for millions of patients living in the nation’s rural communities, the White House issued an executive order on Aug. 3 that calls on the Department of Health and Human Services (HHS) to report on ways to eliminate regulatory burdens. The American Association of Nurse Anesthetists (AANA) is encouraged by the order which, if considered, could increase access to quality care for patients by removing costly supervision requirements of nurse anesthetists.

Showing results

110 of 2835