Credit: Image courtesy of Genome Research
In a study published on the cover of the April 2015 edition of Genome Research, a team including DOE JGI and Berkeley Lab researchers compared two ways of using the next generation Illumina sequencing machines, one of which--TruSeq Synthetic Long-Reads--produced significantly longer reads than the other. Metagenome data were generated from the Berkeley Lab-led DOE subsurface biogeochemistry field study site in Rifle, Colorado by a Banfield-led team. They evaluated the accuracy of the genomes reconstructed from the sequences produced by the two Illumina technologies to learn more about the microbes present in lower amounts than others and better determine the species richness of the metagenome samples.