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Media Contact:
Dr. Jean Beagle Ristaino, 919/515-3257 or [email protected]
Tim Lucas, News Services, 919/515-3470 or [email protected]
Feb. 24, 2000
Extracted DNA May Reveal Cause of Great Irish Potato Famine
FOR IMMEDIATE RELEASE
One of modern science's most baffling mysteries may soon be solved by a North Carolina State University scientist and the tiny fragments of DNA she's extracting from dried potato leaves.
Dr. Jean Beagle Ristaino has extracted strands of DNA from potato leaves preserved from the great Irish potato famine of the 1840s.
Using molecular tools only recently available, Ristaino, a professor of plant pathology at NC State, has decyphered a fragment of DNA she's collected from blighted leaves stored as specimens at the Royal Botanic Gardens in Kew, England, and other famous herbariums.
Her findings should help historians and epidemiologists identify, once and for all, the source of the potato late blight pathogen that decimated Irish potato harvests from 1845 to 1849, killing more than 1 million people and forcing several million more to flee the island nation.
Ristaino made a poster presentation about her findings at the 2000 annual meeting of the American Association for the Advancement of Science in Washington, D.C., last week. This is the first time a disease pathogen has been identified using DNA from a dried plant sample.
"There are so many unsolved mysteries this DNA should finally help us answer," she says. "Where did the late blight pathogen originate? How did it spread around the world? How has it evolved over the past 150 years? Are its new genotypes, or genetic forms, different from the old ones?"
The answers to those questions have more than just historical significance, she says, because the modern form of the late blight pathogen, Phytophthora infestans, is still wiping out potato crops worldwide today, despite modern fungicides and biosafety measures. Outbreaks occur each year in Mexico, Ireland, Ecuador, North Carolina and other potato growing regions of the United States.
"Ultimately, if we can learn how the late blight pathogen has evolved, we can develop new control measures that could help eradicate future outbreaks," Ristaino says.
To conduct her research, Ristaino extracted and amplified ribosomal DNA fragments, 100 base pairs long, from 20 blighted potato leaf specimens. The amplified DNA from the eight oldest specimens known to be infected with the late blight pathogen was sequenced, allowing Ristaino to identify for sure that the pathogen involved was late blight. Next, she plans to identify its genotype and track its historic route of migration around the world.
She has devised new diagnostic tools, using recombinant DNA techniques, that allow more rapid and accurate detection of the pathogen's presence in potatoes before they are stored or planted. NC State has been issued a patent on Ristaino's technique.
Her research collaborators are Greg Parra of NC State and Dr. Carol Trout Groves, a former NC State postdoctoral fellow now at the USDA Agricultural Research Service's New England Plant, Soil and Water Laboratory in Orono, Maine. Their study is funded in part by the National Geographic Society.
Potatoes are one of the world's four leading food crops, along with rice, maize and wheat. Late blight poses an especially great threat to potatoes in developing countries, where control measures may be too costly to use.
"This research opens a window to the past," Ristaino says. "We're excited about our results and believe these kinds of studies of epidemics could be constructed for a range of plant pathogens."
-- lucas --
Editor's note: Ristaino's AAAS poster abstract follows. For the full poster text, contact Tim Lucas at 919/515-3470 or [email protected].
PCR Amplification of Phytophthora infestans From 19th Century Herbarium Specimens
By: J.B. Ristaino and G.R. Parra, North Carolina State University; and C. Trout Groves, New England Plant, Soil and Water Laboratory, USDA, ARS, Orono, ME.
Presented at: American Association for the Advancement of Science annual meeting, Feb. 17-22, 2000, Washington, D.C.
Abstract: Late blight epidemics caused by Phytophthora infestans led to the Irish potato famine in the 1840s. Dried potato and tomato lesions containing the late blight pathogen P. infestans were sampled from the Farlow Herbarium, Harvard University, the Royal Botanic Gardens Mycological Herbarium, Kew, England, and the USDA National Fungus Collections, Beltsville, MD. Protocols for DNA extraction and PCR amplification were optimized using modern herbarium specimens. Various modifications of a CTAB extraction protocol including column purification and ethanol precipitation were effective for obtaining PCR quality DNA from herbarium specimens. A new PCR primer called HERBI used in combination with PINF, a P. infestans specific primer, successfully amplified a 100 base pair fragment of the ribosomal DNA from 20 of 66 herbarium samples tested to date. We have successfully amplified pathogen DNA from an Irish specimen collected by David Moore in 1846 and British samples collected by John L
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