Human branching cholangiocyte organoids recapitulate functional bile duct formation

The intrahepatic bile ducts consist of a complex branching morphology. Here, Roos et al. demonstrate that a branching tubular architecture can be induced in human adult intrahepatic cholangiocyte organoids. These BRCOs faithfully recapitulate aspects of in vivo branching organs and can be used to study bile duct development.