Single-cell analysis of embryoids reveals lineage diversification roadmaps of early human development

Fu and colleagues profiled a stem cell-derived microfluidic amniotic sac embryoid (μPASE) using single-cell RNA sequencing. Molecular maps of lineage diversifications from the pluripotent human epiblast toward the amniotic ectoderm, primitive streak/mesoderm, and primordial germ cells were constructed and compared with in vivo primate data.