Fig. 1. Overview of the developed miRNA detection system with microfluidic device.
Newswise — In natural environments, plants encounter biotic and abiotic stresses that can significantly affect their productivity and health. Recognizing the importance of timely stress diagnosis, researchers have developed various sensors and devices to detect plant hormones, heavy metal responses, and pathogen invasions. Current research is focused on miR399, a microRNA that is critical for phosphate homeostasis in plants, which could serve as a general biomarker for detecting phosphorus starvation. Despite the availability of various miRNA detection methods such as northern blotting, microarray, and real-time PCR, these methods are often time-consuming and complex. The development of microfluidic technology for point-of-care (POC) applications shows promise for rapid, simple, and sensitive miRNA detection, potentially revolutionizing plant stress diagnosis and management. However, there are still challenges in adapting these technologies for widespread agricultural use, highlighting the need for further research to make these diagnostic tools more accessible and effective for precision farming.
In March 2024, Plant Phenomics published a research article entitled by “Microfluidic device for simple diagnosis of plant growth condition by detecting miRNAs from filtered plant extracts”.
To tackle the issue of identifying microRNAs (miRNAs) in plants, a novel microfluidic device system has been developed. The system focuses on detecting miR399, which is a biomarker for phosphorus deficiency stress. The device employed micro-flow channels to immobilize DNA on glass surfaces, facilitating the creation of DNA-probed areas for miRNA detection through sandwich hybridization. The method detected artificially synthesized miR399c, demonstrating the device's high specificity in identifying miRNA presence, even at low concentrations. Further experiments validated the device's effectiveness in detecting miR399c within tomato leaf extracts. Enhancements were made through the use of RNase inhibitors, DTT buffers, and size-fraction filtration to improve detection sensitivity.
Subsequent studies expanded the device's application to detect endogenous miRNAs in tomatoes, revealing an increase in sly-miR399 expression under phosphorus-deficient conditions. The study demonstrated the device's potential for rapid and accurate stress diagnosis in agricultural settings, highlighting its ease of use and the possibility for early intervention in crop management. The microfluidic device's capacity to diagnose phosphorus deficiency stress in tomatoes by detecting sly-miR399 expression was further demonstrated in cultivation experiments. These experiments showed that the device could effectively identify phosphorus deficiency, enabling timely adjustments in nutrient provision to prevent growth failure.
In conclusion, the development and application of this microfluidic device represent a significant advancement in the field of plant phenomics. It offers a practical tool for the early diagnosis of nutrient deficiency stress. The device’s simplicity, accuracy, and potential for point-of-care diagnostics underscore its value in enhancing sustainable agriculture and precision farming, by facilitating early intervention and informed cultivation management decisions.
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References
Authors
Yaichi Kawakatsu1,†, Ryo Okada2,†, Mitsuo Hara3,†, Hiroki Tsutsui2, Naoki Yanagisawa4,5, Tetsuya Higashiyama4,5,6, Akihide Arima7, Yoshinobu Baba7,8,9, Ken-ichi Kurotani1, Michitaka Notaguchi1,2,10,*
†These authors contributed equally to this work.
Affiliations
1Bioscience and Biotechnology Center, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
2Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
3Department of Molecular and Macromolecular Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8603, Japan.
4Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601 Japan.
5Institute of Transformative Bio-Molecules, Nagoya University, Nagoya, 464-8601 Japan.
6Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
7Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
8Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
9Institute of Quantum Life Science, National Institutes for Quantum Science and Technology (QST), Anagawa 4-9-1, Inage-ku, Chiba 263-8555, Japan.
10Department of Botany, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
About Michitaka Notaguchi
Designated Professor, Graduate School of Bioagricultural Sciences, Nagoya University. He is working on the mechanism of plant grafting and systemic signal transduction.
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