Symptoms of the crown rot disease of strawberry caused by Macrophomina phaseolina. The strawberry plants depicted here are selection cycle one progeny observed August 30, 2022 in Salinas, CA. The ordinal scores applied to visual symptoms are shown below the photographic images, where 1 = highly resistant (symptomless) and 5 = highly susceptible (dead).
Newswise — Globally, strawberries are gravely affected by Macrophomina phaseolina, a soilborne fungal pathogen that drastically reduces yields. Following the phase-out of conventional soil fumigants like methyl bromide, there is an urgent need to enhance genetic resistance to this pathogen. The resistance mechanism is intricate at the genetic level, presenting considerable obstacles to traditional breeding methods. Consequently, there is a pressing need for in-depth research to devise sophisticated genetic strategies to effectively counter this threat.
The study (DOI: 10.1093/hr/uhad289), conducted by the Department of Plant Sciences at UC Davis and published in Horticulture Research on January 3, 2024, illustrates how phenotypic selection under stress conditions can swiftly bolster disease resistance in strawberries. Researchers leveraged genome-wide association studies (GWAS) and state-of-the-art genomic tools to unravel the resistance mechanism, identifying several pivotal resistance loci.
This research offers an exhaustive analysis of the genetic resistance to Macrophomina in strawberries, revealing it as a complex polygenic trait influenced by numerous loci. Utilizing advanced genomic technologies, including GWAS, researchers pinpointed these essential resistance loci. This facilitated the effective stacking of favorable alleles, significantly boosting resistance levels in the breeding population from 1% to 74% within two selection cycles. By implementing genomic selection techniques and simulating environmental stress conditions during the breeding process, researchers achieved rapid genetic improvements. The application of high-throughput genotyping and strategic allele stacking enhanced the identification and utilization of key genetic elements, refining the breeding strategy for the rapid development of resistant strawberry varieties.
Dr. Steven J. Knapp, the study's lead author, stated, "This research not only propels our understanding of genetic resistance forward but also establishes a model for predictive breeding approaches that could be employed for other crops facing similar challenges."
The implications of this study reach beyond strawberries, providing a template for addressing diseases in other crops via genomic selection. This method could transform how breeders manage disease resistance, diminishing reliance on chemical treatments and boosting crop sustainability. Additionally, the cultivation of disease-resistant strawberry varieties could greatly enhance both yield and quality, offering substantial benefits to growers and consumers alike.
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References
DOI
Original Source URL
https://doi.org/10.1093/hr/uhad289
Funding information
This research was supported by grants to SJK from the United Stated Department of Agriculture (http://dx.doi.org/10.13039/100000199) National Institute of Food and Agriculture (NIFA) Specialty Crops Research Initiative (SCRI) (#2017-51181B6833), SJK, MJF, DDAP, MB, and PMH from the USDA NIFA SCRI (#2022-51181-38328-0), and SJK, GSC, MJF, DDAP, and MB from the California Strawberry Commission (http://dx.doi.org/10.13039/100006760).
About Horticulture Research
Horticulture Research is an open access journal of Nanjing Agricultural University and ranked number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. The journal is committed to publishing original research articles, reviews, perspectives, comments, correspondence articles and letters to the editor related to all major horticultural plants and disciplines, including biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.
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